Instruction Manual – 11
9. Checking Pipetting Accuracy Parameters
and Pipette Recalibration
The EPRO® Sailor™ pipettes have been factory-calibrated
using gravimetric methods with manufacturer pipette tips
and distilled water, in accordance with ISO 8655 guidelines
for the maximum (nominal) liquid volume drawn by the
pipette and for 10% of the maximum or minimum liquid
volume according to the values given (Section 5).
The EPRO® Sailor™ pipettes are designed to enable
recalibration and adaptation to different pipetting
techniques and liquid properties (e.g., temperature,
density, and viscosity).
Periodic checks of the operation of the pipette are
recommended at least once per year. Frequency of checks
should be increased depending on workload, sterilization
or autoclave processes, and frequency of replacement of
component parts.
If during pipette operation the accuracy error (the
difference between the real aspirated volume and the
preset volume) exceeds the permissible value given
in the table in Section 5, pipette recalibration should be
carried out.
Recalibration of the pipette involves volume adjustment
based on the value obtained by weighing. Recalibration of
the pipette is performed only for one liquid volume drawn
by the pipette.
The recommended volume for recalibration is 10% of the
maximum (nominal) value or minimum volume depending
on which of these values is greater. See table on page 13 for
more details.
