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Single Channel Pipettes

Instruction Manual

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Summary of Contents for Sailor

Page 1: ...Single Channel Pipettes Instruction Manual ...

Page 2: ...commendations 3 5 Specifications 4 6 Pipette Operation 5 7 Operating Instructions 8 8 Aspiration and Dispensing Instructions 9 9 Checking Pipetting Accuracy Parameters and Pipette Recalibration 11 10 Pipette Maintenance 14 11 Troubleshooting 15 12 Spare Parts 17 13 Limited Warranty 19 ...

Page 3: ...ed 10 EPS10 0 5 10 20 EPS20 2 20 Yellow 200 EPS200 20 200 1 000 EPS1000 100 1 000 Blue The EPRO Sailor pipettes operate using an air cushion i e the aspirated liquid does not come into contact with the shaft or plunger of the pipette The liquid is drawn into the disposable tip attached to the pipette 2 Packing The pipettes are delivered with the following Description Qty Pk Instruction manual QR c...

Page 4: ...stment knob B1 Locking ring B2 Color identification ring P Shaft C Tip ejector D Shaft nut F A1 A2 A Pipetting pushbutton Consists of 2 parts the pushbutton A1 and the knob A2 B1 Volume adjustment knob Used to set the volume B2 Locking ring Allows the volume setting to be locked by pushing the ring upwards C Shaft Manufactured using high quality plastic ensuring high chemical and mechanical streng...

Page 5: ... tip Only using the pipette in accordance with the manufacturer s instructions ensures the correct pipette parameters are maintained After replacing the plunger or the shaft the pipette should be calibrated In the case of incorrect operation the device should be cleaned in accordance with the Instructions for use or transferred to a service point Ambient operating temperature is 5 C to 45 C Ambien...

Page 6: ... filter Tips μL 2 Min 0 1 40 0 12 0 10 0 2 12 0 6 0 1 2 7 1 3 Max 2 1 5 0 7 10 Min 0 5 4 0 2 8 10 1 0 2 5 1 8 5 0 1 0 0 6 Max 10 0 0 5 0 4 20 Min 2 3 0 1 5 200 10 1 0 0 5 Max 20 0 8 0 3 200 Min 20 1 2 0 60 200 100 0 8 0 25 Max 200 0 6 0 20 1 000 Min 100 1 6 0 40 1 000 500 0 7 0 20 Max 1 000 0 6 0 15 The accuracy and precision were obtained gravimetrically using manufacturer tips performing at leas...

Page 7: ...ion to ensure an airtight seal NOTE Do not attach the tips with a rocking movement as this may damage the shaft or plunger Observe this rule particularly with single channel pipettes of low volume range Never draw liquids directly into the pipette without the tip attached Adjusting the tip ejector The tip ejector can be adjusted by the user and can accommodate most types of tips available on the m...

Page 8: ... arbor 2 Tip ejector assembly Press the tip ejector pushbutton Line the metal tip ejector up with the plastic arbor Turn the metal tip ejector clockwise until it has fully latched 3 Tip ejector adjustment Remove the tip ejector pushbutton To increase the ejector length turn the plastic bush counter clockwise To decrease the ejector length turn the plastic bush clockwise ...

Page 9: ...tting pushbutton knob or the adjustment knob The volume setting is displayed as a three digit counter which should be read from top to bottom The smallest volume increment is printed on the bottom counter drum 3 After the volume has been set set the locking ring to the upper position to avoid inadvertent changing of volume Examples of counter indications The decimal point in the volume of aspirate...

Page 10: ... depth which should remain constant during aspiration The recommended immersion depths are given in the table below Model Volume Range µL Immersion Depth mm 0 1 1 1 1 100 2 3 101 1 000 2 4 The pipette should be held in a vertical position The pipette tip should be changed whenever the volume setting is altered and when a different liquid is to be aspirated The pipette tip should be changed if a dr...

Page 11: ... the pipetting operation 8 Aspiration and Dispensing Instructions 1 2 3 5 4 6 Aspirating Liquid 1 Press the pushbutton to the first stop point Holding the pipette vertically immerse the pipette tip into the sample liquid to the recommended depth for recommended values see Section 7 If the pipette tip is not immersed to the recommended depth or if the pipetting pushbutton is rapidly released air ma...

Page 12: ... As the film remains relatively constant in successive pipetting operations with the same tip this error can be eliminated by pre rinsing the tip and allowing a film to form before transferring the first sample This is achieved by aspirating a sample and dispensing it back into the same vessel Allowing a film to form prior to sampling ensures optimal accuracy and repeatability This pre rinsing ope...

Page 13: ...he operation of the pipette are recommended at least once per year Frequency of checks should be increased depending on workload sterilization or autoclave processes and frequency of replacement of component parts If during pipette operation the accuracy error the difference between the real aspirated volume and the preset volume exceeds the permissible value given in the table in Section 5 pipett...

Page 14: ... be measured Volume Checked V μL Balance Sensitivity mg 0 1 V 10 0 001 10 V 100 0 01 100 V 1 000 0 1 When calculating the liquid volume aspirated by the pipette the conversion factor Z μL mg for distilled water or a liquid with comparable density should be taken into account Sample values of conversion factors are given in the following table Temperature C Pressure kPa 95 0 101 3 105 0 20 1 0028 1...

Page 15: ...ersion factor Z μL mg If the average aspirated volume exceeds the permissible values of the range the pipette should be recalibrated Pipette Recalibration K 1 Remove the pipetting pushbutton CAUTION The pipetting pushbutton consists of 2 parts the knob and the pushbutton After removal of the pushbutton both parts are separated 2 Holding the volume setting knob to prevent rotation insert the calibr...

Page 16: ...leaned Do not use sharp tools for pipette maintenance It may cause damage to the device and affect the user s safety Cleaning External surfaces of the pipette such as the pushbutton ejector pushbutton handgrip shaft nut and adjustment knob may be cleaned using a cloth dampened in isopropyl alcohol The remaining parts removed from the pipette during pipette disassembly may be washed with distilled ...

Page 17: ...can cause discoloration of pipette parts but does not affect its performance 11 Troubleshooting If a problem is encountered during pipette operation use the following table to identify and eliminate the fault following the instructions provided Replacement of parts should be required only occasionally and should not be required with normal pipette use Problem Cause Solution Droplets of liquid rema...

Page 18: ...shbutton gets blocked The inside of the pipette is contaminated due to aspiration of corrosive substances Unscrew the shaft set wash the parts Replace the elements with new ones if necessary Apply a small amount of lubricant on the plunger and reassemble the set in the correct order The inside of the pipette is contaminated due to the liquid entering the pipette The sealing elements are not suffic...

Page 19: ... NOTE The replacement of the plunger requires conducting the calibration procedure according to Section 9 I J G G B F P C D M S Item Description Model Cat No Qty Pk B Volume adjustment knob set All ESP20221 1 C I J Shaft with O ring and seal 2 ESP20222 1 10 ESP20223 1 20 ESP20224 1 200 ESP20225 1 1 000 ESP20226 1 D Tip ejector 2 10 ESP20211 1 20 ESP20212 1 200 ESP20213 1 1 000 ESP20214 1 Seal J us...

Page 20: ...ESP20216 1 20 ESP20217 1 200 ESP20218 1 1 000 ESP20219 1 I J O ring and seal 2 ESP20227 10 10 ESP20228 10 20 ESP20229 10 200 ESP20220 10 1 000 ESP20230 10 K Calibration key All ESP20231 1 M Tip ejector cap 2 10 ESP20232 3 P Color indentification rings All ESP20233 6 S Wall mount All ESP20234 1 Seal J used only in 2 µL model ...

Page 21: ...e original material workmanship This warranty does not cover the O ring or shaft Claims for transit damage should be filed with the transportation carrier In the event this product fails within the specified period of time because of a defect in material or workmanship contact EPRO Customer Service at tenders eproscience com visit www eproscience com or contact your local support office EPRO Custo...

Page 22: ..._____________________________________ Supplier _________________________________________________ Warranty Disclaimer Unless otherwise specified all products are for research use only Not intended for use in diagnostic or therapeutic procedures EPRO makes no claims regarding the performance of these products for clinical or diagnostic applications This manual is available in other languages at http...

Page 23: ...t information or technical information visit www eproscience com or contact your local sales office EUROPE office eproscience com 48 797 866 390 2021 EPRO All rights reserved 11 21 EPRO AN 1000DOC REV2 Made in Poland For a listing of trademarks visit www eproscience com All other trademarks are the property of their respective owners ...

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