Eicom ENO-30, User Manual

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release to “pop” the syringe. Then close the valve and check for symptoms again. Repeat a
couple of times.
3) The next option is to pull a large bubble through the pump head. Lift the solution inlet tubing
out of the liquid and aspirate at the purge valve until there is a large bubble in the tubing. Lower
the inlet back in the reservoir and aspirate until the air comes into the syringe.
4) The next method is to force liquid through the pump head by connecting a syringe to the
three way joint at the bottom of the pump head. There are details in video available on the
Eicom users’ site or directly from your Eicom representative.
13-4. High Carrier Pump pressure
Check the guard column. Remove the guard column and replace it with a two way. Check the
pressure. If the pressure is standard pressure, the guard column should be repacked (see
chapter 17).
13-5. Unstable Baseline

Has the system run for more than 30 minutes? It takes time for the system to stabilize and
the oven to get to temperature.

Have you check the pump flow rates? Maybe there is air in one of the pumps.

Are there any leaks at the tubing connections?
13-6. Peak Shape
Is the peak strange, asymmetry, or tailing? The peak shape is influenced mainly by the
separation column and guard column. See chapters 10 and 11.