Eicom ENO-30, User Manual

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10-2. How to Repack the Precolumn
1) Turn off the reactor pumps and then turn off the carrier pumps.
2) Remove the guard column and replace with a two way joint.
3) Open the precolumn using two wrenches. Then remove the old packing material and wash
out the inside of the empty column.
Flow
4) Prepare slurry of packing gel by adding about 30 mL of pure methanol to the bottle that the
packing gel came in.
The exact amount of methanol isn’t important. If the bottle has been
used before, the methanol may have evaporated. Just add some more, fresh methanol
based on the amount of packing material still in the bottle. Cap the bottle and shake. The gel
will not dissolve.
5) Connect the 2.5 mL syringe to the lower half of the
precolumn via a syringe union and a piece of PEEK tubing.
6) Use a 20-200 µL wide bore pipette (or a normal pipette tip