Eicom ENO-30, User Manual

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6-8. Start up
1. Turn on the power switches for the Detector/Oven and Pump Unit. (located on the left side)
The oven will start to heat to 35 °C. The pump display with light and you will hear the
degasser start
, but the pumps won’t start yet.
2. Turn on autosampler power. Read panel on left side. You will hear the tray move back and
forth as it initializes. The green light at the front will illuminate.
3. Run autosampler initial wash cycle.
4. Use the key pad to set the Carrier pump to 0.33 mL/min and start the Carrier pump first.
You may want to start it at a higher rate (0.5 mL/min) to fill the lines and wash out the lines,
but reset to 0.33 mL/min for analysis.
5. Now set the Reactor pump to 0.1 mL/min and start the pump. Again you can use a higher
setting initially if you prefer.
6. Confirm flow rates are correct by collecting fluid at the waste line. Run each pump
separately. Remember the columns should not be installed yet. Collect waste into small
microcentrifuge tube for one minute. If it is half the expected volume, then the pump has a
bubble and needs to be primed again to remove.
7. Confirm that the Reactor and Carrier solution are routed to the right pumps. If you install
the columns when the Reactor and Carrier solution are routed to the wrong pumps, the
columns will suffer serious damage. To avoid problems PLEASE BE AWARE OF
FOLLOWING:

DO NOT put Reactor tube in Carrier bottle.

DO NOT put Carrier tube in Reactor bottle.

DO NOT run the Reactor pump while the Carrier pump is OFF.

When REAC. PUMP is ON, DO NOT disconnect the line from Carrier pumps.

When REAC. PUMP is ON, DO NOT open the purge valve.
8. Now you can install the columns. Turn off the pumps. Replace the two way joint with the
columns and restart the pumps.
9. Start the Envision software, and monitor the detector signal in the Acquisition Monitor until
it stabilizes. (30-60 mins)
10. Inject 10 µL of 10 µM standard solution a calibration chromatogram. The sensitivity of
ENO-30 must be check by a standard solution before each analysis. The peak height of
NO
2
-
should be over 16 mV (10 µL

10 µM= 100 pmol).