Eicom ENO-30, User Manual

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6-4. Carrier and Reactor Solutions Preparation
For analysis of NO
2
-
and NO
3
-
, the Carrier and Reactor should be prepared using Eicom Carrier
Powder (NO-CAP3) and Reactor A and B powders (NO-RAP3 and NO-RBP3). Reactor A and B
solution are made separately and just enough for one day’s analysis is prepare by mixing 1:1.
Carrier Solution
Add 900 mL of water to a 1 liter glass bottle. Add 100 mL of methanol to the bottle. Pour in
Carrier powder. Shake to dissolve. Rinse the bottle the powder came in with the prepared
Carrier solution to dissolve last of powder and return to prepared solution.
Reactor A
Add 450 mL of water to a 500 mL bottle. Add 100 mL of methanol to the bottle. Add 12.5 mL of
concentrated HCL (35-57%). Pour in Reactor A Powder. Shake to dissolve. Rinse the bottle the
powder came in with the prepared Reactor A solution to dissolve last of powder and return to
prepared solution.
Reactor B
Add 450 mL of water to a 500 mL bottle. Add 100 mL of methanol to the bottle. Pour in Reactor
B Powder. Shake to dissolve. Rinse the bottle the powder came in with the prepared Reactor B
solution to dissolve last of powder and return to prepared solution.
Complete Reactor Solution
Mix equal parts of Reactor A and B on the day of the analysis.
Storage
Carrier Solution can be stored for several months in the refrigeration. Do not run the system with
cold solution because air bubbles can form in the HPLC pumps and cause them to stop.
Complete Reactor solution can only be used for one or two days. Only mix enough for the day.
The solution can absorb NO
2
from the air and turn pink. Also cover the bottle with aluminum foil
to protect it from light. If the Reactor has turned pink, it cannot be used for high sensitive
analysis. If the Reactor is stored in a transparent bottle, it is easier to judge any color change.
Do not allow any of the Reactor to enter into columns. Columns will suffer serious damage from
contact with the Reactor.
Reactor A and B can be stored for several months in the dark at room temperature or in the
refrigerator, but the longer they sit, the low the potency of the complete Reactor solution when
it’s prepared. It will be immediately pink, leading to lower sensitivity analysis.