
Revision: JUNE 2017
65
Stick the needle down into the HPLC tubing connection of the cone and depress the hypodermic syringe filling the open
area inside, displacing the air. As the air is replaced, use the needle to help remove air bubbles or pockets, using the
velocity of the water moving out of the hypodermic needle. Once you have filled the open area of the compression fitting
with water and have removed all the air, pull the hypodermic needle out while still depressing water out to fill the displace
-
ment of the needle itself.
Make sure that you have water on top of the open area of the HPLC connection with no air pockets below.
9. Note the tubing you are using. If you are using the smaller 1mm ID “Natural” HPLC tubing recommended for use with
small compression coupling (1-20mm),then you will need two large viton “O” rings with one small viton “O” ring for con-
necting the tubing to the compression coupling. If you are using the larger 1.5mm Teflon clear tubing, you will only need
the two larger “O” rings for connecting with larger couplings (19-50mm).
Photo 13
Removing Air from the Coupling
“O” Ring Layout Diagram
Summary of Contents for HPFM3
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