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Gentier Real-Time PCR System
A. Overview
1. Application Fields
The Gentier real-time PCR system is intended for performing rapid, accurate polymerase chain
reaction (PCR), meanwhile real-time measuring the nucleic acid signals from DNA-binding
fluorescent dyes or labeled probes and converts them to comparative quantitative readouts of
DNA or reverse transcribed RNA.
The Gentier real-time PCR system can be used in medical institution laboratories and clinical
laboratories for infectious pathogens (such as viruses, bacteria, mycoplasma, chlamydia, etc.)
detection; or multiple tumor marker tests of neoplastic diseases, etc. For scientific research, it
could be used for the fluorescence quantitative or qualitative analysis of genetic materials
DNA/RNA in fields of immunology, molecular biology, forensic science, genetics, archeology,
zoology, phytology, etc.
2. Product Constitute and Instrument Structure
2.1 Product Constitute
The Gentier real-time PCR system is mainly consisted of control system, power system,
temperature control system, detection system, man-machine interface system and instrument
shell, etc.
Among them, the temperature control system includes Peltier heating module, sample block and
hot lid, etc; the detection system includes LED excitation light source, photodiodes and light
filter, etc; the man-machine interface system includes data acquisition and analysis software,
which is mainly responsible for real-time data collection, fluorescence diagram formation, data
processing and diagram analysis, in order to quantify or characterize the target nucleic acid and
obtain other test report information, etc.
2.2 Instrument Structure
The structure of Gentier instrument is shown in figure A-1.