112
Gentier Real-Time PCR System
3.
▶
a.
.
Run Monitoring Diagram
:
If user select the
Fluorescence
option in the run monitoring option bar, user can press
or
keys below the run monitoring diagram to choose the monitor content, which can
be the real-time
Amlificaiton
curve
, Melting
curve or fluorescence
Heat Map
of the
current experiment, as shown in figure E-5.1.
The run monitoring diagram will display the real-time
Amlificaiton
curve of the current
experiment by default, as shown in figure E-5.1.
Figure E-5.1 Run Monitoring Diagram - Real-time Amplification Curve
X axis represents the cycle number.
Y axis represents the Relative Fluorescence Unit (RFU).
User can press any dye under the
Fluorescence
option in the run monitoring option bar,
and the run monitoring diagram will only display the corresponding real-time
amplification curve.
User can press
key below the run monitoring diagram to monitor the real-time
Melting
curve of the current experiment, as shown in figure E-5.2.
X axis represents the temperature.
Y axis represents the Relative Fluorescence Unit (RFU).
User can press any dye under the
Fluorescence
option in the run monitoring option bar,
and the run monitoring diagram will only display the corresponding real-time melting
curve.
b
