7
Introduction
The “DTprime” Detecting Thermocycler (hereafter – the “instrument” or “device”) is
intended to conduct qualitative and quantitative PCR analysis dropping out electrophoresis
stage of PCR products in agarose gel when using PCR kits based on the fluorescent detection
principle. Measurement of the quantity of accumulated product of PCR amplification takes
place right in the course of reaction temperature cycles (real-time PCR). The qualitative
analysis is based on evaluation of kinetics of PCR visible part, carried out using relevant
mathematical tool.
The use of fluorescent detection of PCR products has a list of substantial advantages:
high detection specificity (if oligonucleotide probes are applied, which allow detecting
only of certain amplicons);
high efficiency;
reduced assay time;
carrying out detection in a closed tube, which practically eliminates contamination of
further experiments;
availability of quantitative assessment of initial DNA-matrix;
registering and accounting of the data in electronic format.
Fig.1. Instrument General View
