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1.3 ORGAN BATH CHAMBER - 820MO

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Figure 1.4 Close up of the 820MO myograph chamber unit

Figure 1.3 820MO Organ bath chamber unit and 820MO-XL Chamber Unit

Connection to Organ Bath Interface

Micrometer positioner

Organ bath clamp support
connected to micrometer

Force transducer pin

Organ bath clamp support
connected to force transducer

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Summary of Contents for 820MO

Page 1: ...ORGAN BATH SYSTEM 820MO USER MANUAL VOL 1 2 ...

Page 2: ...as accurate at the time of printing Since then changes may have been made to the software and hardware it describes New information may be supplied separately This documentation is provided with the DMT Organ Bath System Model 820MO Document Number 820MO UG2 2A All rights reserved No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of ...

Page 3: ...System 820MO 10 2 2 Setting up step by step 11 CHAPTER 3 THEINTERFACE MENUS 13 3 1 Interface Menus Navigation 13 3 2 Interface Menus Calibrated Mode 16 CHAPTER 4 THE ORGAN BATH CHAMBER UNIT 28 4 1 Calibration of theForce Transducer 28 4 2 Checking the Force Transducer 30 4 3 Force Transducer Replacement 31 4 4 Organ Bath Maintenance 33 4 5 Cleaning the System 34 CHAPTER 5 MOUNTING SUPPORTS AND MUS...

Page 4: ... AND ACCESSORIES SPECIFIED BY THE MANUFACTURER UNPLUG THIS APPARATUS DURING LIGHTNING STORMS OR WHEN UNUSED FOR LONG PERIODS OF TIME BE ADVISED THAT DIFFERENT OPERATING VOLTAGES REQUIRE THE USE OF DIFFERENT TYPES OF LINE CORD AND ATTACHMENT PLUGS CHECK THE VOLTAGE IN YOUR AREA AND USE THE CORRECT TYPE SEE THE TABLE BELOW Voltage Line plug according to standard 110 125V UL81 and CSA C22 2 No 42 220...

Page 5: ...T immediately and the matter will be pursued as soon as possible If the packing material appears damaged please retain it until a potential claim has been settled We recommend that you store the packing material for any possible future transport of the Organ Bath System In case of transportation and the original packing material is unavailable please get in touch with DMT for advice and packing in...

Page 6: ...EMC Directive 2004 108 EC Based on the following specifications applied by EN 61326 1 2006 EN 61326 2 6 2006 EN 61326 2 6 Corr 2007 And with the LVD Directive 2006 95 EC Based on the following specifications applied by EN 61010 1 2010 EN 61010 2 030 2010 General warnings regarding EMC Do not use this device in close proximity to sources of strong electromagnetic radiation e g unshielded intentiona...

Page 7: ...CHAPTER 1 SYSTEM OVERVIEW 1 1 INTERFACE 7 Myo Interface display Heat indicator Power indicator Valve buttons Figure 1 1 Interface front panel ...

Page 8: ...nection pH meter connection Transducer connections Gas regulator needle valve Gas input Vacuum input ON OFF switch 4 Recorder outputs analogue USB output digital Grounding connected to Data Acquisition Power connector Figure 1 2 Interface Rear Panel ...

Page 9: ...ograph chamber unit Figure 1 3 820MO Organ bath chamber unit and 820MO XL Chamber Unit Connection to Organ Bath Interface Micrometer positioner Organ bath clamp support connected to micrometer Force transducer pin Organ bath clamp support connected to force transducer ...

Page 10: ...CHAPTER 2 SETTING UP 2 1 SETTING UP THE MUSCLE STRIP MYOGRAPH SYSTEM 820MS 10 Figure 2 1 The complete Organ Bath System 820MO ...

Page 11: ...ygen Supply Connect the gas supply 95 O2 5 CO2 or 21 O2 5 CO2 balance N2 with tubing running from the gas supply to the gas inlet MARKED OXYGEN on the back of the Interface This stainless steel inlet pipe will supply oxygen to the chambers The oxygen tubing needs to be inserted into the chamber in order to aerate the heated buffer Needle valves on the back of the Interface can be adjusted to regul...

Page 12: ...igure 2 4B Chamber cover with built in electrodes optional item 5 Initial Force Transducer Status Prior to the shipment of the Organ Bath 820MO System it has gone through two days of continuous testing including a final force transducer calibration However DMT recommends that a new force transducer calibration is performed before using the organ bath system for the first time The force transducer ...

Page 13: ...ng can be changed by touchingthe SELECT icononthescreencorrespondingto the SELECT icon on the touch screen corresponding to the desired channel to be changed The line to be modified will turn blue indicating that the Interface is waiting for input When ALL is chosen all lines corresponding to all 4 channels will turn blue Changing the numeric value for the chosen parameter can be done by touching ...

Page 14: ...n appears The system is auto calibrating the A D converters while this screen is displayed After a few seconds the ACTUAL FORCE display will appear At any given time if the force applied on any channel is out of range the force reading for the overloaded channel will turn yellow as a warning 14 ...

Page 15: ...ion and return the user to the ACTUAL FORCE display HEAT MENU The heating unit and temperature are controlled from this menu To turn the heat on or change the preset temperature for the system access the temperature control menu Pressing the HEAT key will enter the menu and allow the user to change the default system temperature as well as turn the heat on or off Pressing DEFAULT will automaticall...

Page 16: ...ressing FORCE CALIBRATION to enter the sub menu The sub menu will list all 4 chambers for calibration For more information and tips please refer to Section 4 1 Calibration of the Force Transducer Tobeginthecalibration press SELECT forthechamberwhich calibration will be performed on The text for the chamber to be calibrated will turn blue Pressing ENTER will enter the 6 step procedure for calibrati...

Page 17: ... close as possible to the mounting pin or clamp withouttouchingtogetthemost accuratecalibration Press NEXT STEP when the calibration kit has been properly placed Step 3 initiates the heating process for the chambers For the calibration to be accurate the transducers must be heated to the experimental temperature to be used to accommodate heat induced expansion of the electronic parts in the transd...

Page 18: ... force The weight placement should cause a positive increase in the 4 digit number Wait at least 30 to 40 seconds for the applied force to stabilize before pressing NEXT STEP Once the 4 digit number has stabilized press NEXT STEP Step 6 is to verify that the calibration was performed correctly The Force Chamber 1 reading should be 9 81 0 1mN If the Force Chamber 1 reading is off by more than 0 1mN...

Page 19: ...turn on stirring of the high buffer solution When the relative pH output in the blue line is stable go to NEXT STEP Step 3 Place the pH electrode and temperature probe in the low buffer solution here pH 4 and turn on stirring of the low buffer solution When the relative pH output in the blue line is stable go to NEXT STEP 19 NOTE BEFORE THE PH CALIBRATION IS PERFORMED BE SURE TO SELECT THE WAY THE...

Page 20: ...0mN and will appear as a flat line trace at 20mN even though the force readings on the Interface may exceed 20mN Therefore change the FORCE REC OUT settings to an appropriate setting to capture any maximal response from the mounted tissue of interest This value should not exceed the settings for the transducer range which is defined by the sub menu MEASUREMENT RANGE and is explained in the next se...

Page 21: ...r chambers If the Measurement Range is changed to e g 400mN then the Force Rec Out Put also has to be changed to 400mN After this change users of the analog signal from the BNC connectors must go into the data acquisition software and set 0V equals 0mN and 2 5V equals 400mN For DMT Device Enabler users the only thing to do is to restart LabChart then the new settings are transferred to LabChart 21...

Page 22: ...pressing ENTER The ALL functions are used to change all four chambers Force Rec Output values at the same time Pressing ENTER will store the values in memory for future experiments 22 NOTE FOR DMT DEVICE ENABLER USERS LABCHART HAS TO BE RESTARTED AFTER CHANGING THE MEASUREMENT RANGE VALUES OTHERWISE THE OLD SETTING WILL PERSIST IN LABCHART AND THE FORCE READINGS WILL NOT BE CORRECT ...

Page 23: ... temperature for the system may not heat to the exact defined set point Therefore the user can adjust the temperature of each chamber individually to fine tune the temperature setting so that EXACT temperatures can be achieved for any particular chamber This is referred to as a temperature offset TEMP OFFSET ON CHAMBER Use the thermometer connected to the backside of the 820MO Interface to check t...

Page 24: ...rs can be modified at the same time Again the up and down arrow keys can be used to modify the length of time the vacuum valves stay open Pressing SELECT next to any given channel will cause the line selected to turn blue The up and down arrow keys can then be used to modify the length of time the vacuum valves stay open after the valves have been activated with the push buttons on the front panel...

Page 25: ...r and entered manually in the Manual Temp value line IV Select mN or g The contraction force can be shown in gram or in mN on the touch screen and for LabChart Pro and DMT device enabler users the selected force unit will be shown automatically in LabChart as well For users of Powerlab or similar systems the analog signal from the BNC connectors still has to make the unit conversion in the appropr...

Page 26: ...n or mechanisms to change other settings controlled by the onboard computer VI Myograph Type The Interface of the 820MO system is identical to the interfaces of the DMT 620M Multi Myograph and 630MA Automated Multi Myograph system The difference between these three systems is the chambers This makes it possible to combine the three different types of chambers on this Interface There are small diff...

Page 27: ...tters indicating that this systems menu structure is selected Turn off the power of the system and turn it ON again Now the system will load the menus of the select myograph system into the Interface and is now ready for use It is important to make a new weight calibration and make sure that the Measurement Range is correct If the device enabler is not used then also check the Force Rec Output val...

Page 28: ... sub menu under SETTINGS as explained in Chapter 3 The Interface Menus FORCE TRANSDUCER CALIBRATION PROCEDURE This section contains step by step instructions to calibrate the force transducer CALIBRATION PROCEDURE IN CALIBRATED MODE 1 Move the mounting supports apart Fill the chamber with distilled water or buffer Use the same volume that will be used during the experiments 2 Set up the calibratio...

Page 29: ...arm of the bridge with the pans is not touching any part of the mounting support Go to the main menu displaying the forces and zero the channel being calibrated so the force reads zero Slowly and gently slide the calibration kit forward toward the micromanipulator so that the transducer arm rests on the mounting support creating a force reading on that channel Carefully slide the calibration kit b...

Page 30: ...rface If the force reading s are still unstable or noisy then perform a new calibration of the force transducer as described in Chapter 3 The Interface Menus and 4 1 Calibration of the force transducer During the new calibration monitor the relative force reading values in the FORCE CALIBRATION sub menu on the Interface Steps 4 and 5 of the calibration procedure If the value is 0 a single digit or...

Page 31: ...5 Place a VERY small amount of vacuum grease clear or whitish grease around the bottom inside of the transducer housing to seal the transducer housing when put back in place An arrow in figure 4 5 indicate the place that the grease needs to be applied 6 Carefully realign the transducer housing with the new transducer on the Organ Bath Chamber and reinsert the Allen screws through the bottom of the...

Page 32: ...e transducer housing and close up of transducer pin inside the transducer The arrow indicates the place that the grease needs to be applied to prevent buffer and water from damaging the transducer Figure 4 6 Close up of transducer pin from outside The arrow indicates the place that the grease needs to be applied to prevent water and buffer leaking into the transducer housing and damaging the trans...

Page 33: ...y water or buffer remaining in the chamber or on the tubing using absorbent paper FORCE TRANSDUCER The force transducer is the most delicate and fragile component of the organ bath system Extreme care must be used when handling or touching the force transducers As a part of daily maintenance inspect the grease around the transducer pin extending from the transducer housing pinhole before starting ...

Page 34: ...h experiment use the following procedure to clean the organ bath chambers and supports 1 Fill the organ bath chamber to the edge with an 8 acetic acid solution and allow it to work for a few minutes to dissolve calcium deposits and other salt build up Use a cotton tipped applicator to mechanically clean all chamber surfaces Be careful not to apply too much force to the force transducer side mount ...

Page 35: ...hanol solution and wash a few times with double distilled water Incubate the organ bath chamber and supports with an 8 acetic acid solution for 2 minutes and continue the mechanical cleaning with a swab stick 6 Wash the organ bath chamber and supports several times with double distilled water 7 Dry the surfaces using absorbent paper i e Kim Wipes or cotton tip applicators 35 IMPORTANT BE VERY CARE...

Page 36: ...timally clamp the tissue without slipping during a contraction They are also designed to effectively grab tendon on whole striated muscle preparations without experiencing slippage during a whole striated muscle contraction 1 Starting position 2 Loosen the screws on the mounting clamps 3 Turn the upper wings of the mounting clamps to expose the base of the support 4 Place the muscle strip onto the...

Page 37: ...ount ring preparation with an internal diameter from 450µm and up For large tissue ring preparation with the ability to make high force contractions the 200µm pins may be too thin DMT can delivery mounting pins with the following thickness L shaped mounting pins Diameter 100µm 200µm 250µm 300µm and 400µm Figure 5 1 820MO Chamber unit with Mounting Pins 37 IMPORTANT BE VERY CAREFUL WHEN TIGHTENING ...

Page 38: ... using the micrometer until the force reading is increasing 6 The tissue is now mounted and ready for a normalization protocol see chapter 3 2 finding the optimal pre load tension for the mounted tissue 7 Place the micrometer at the position giving the optimal pre load tension on the mounted tissue 8 Continue with a standard start see chapter 3 3 9 The mounted tissue is now ready for experiments 3...

Page 39: ... the extent of stretch which makes it important to set the preparation to an internal circumference or pre load tension giving maximal response of a given tissue mounted in the 820MO chambers The aim of the normalization procedure is to stretch the mounted muscle segment to an optimal pre load tension giving maximum response by muscle contraction For arteries the so called normalized internal circ...

Page 40: ...sing Individually controlled per chamber by needle valve Force range User selectable at 200 400 800 1600mN Force resolution 0 1mN Weight calibration Semi automatic Heating Built into chamber independent of super fusion Temperature range Ambient temperature 50 C Temperature resolution 0 1 C Temperature probe External Output reading Force mN or Grams g Analogue output Independently filtered or unfil...

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