CHAPTER 4 - THE ORGAN BATH CHAMBER UNIT
Chapter 4 contains a complete explanation of how to calibrate and maintain the 820MO organ bath to ensure the
equipment is always performing at peak performance.
4.1 CALIBRATION OF THE FORCE TRANSDUCER
As a part of the general maintenance of the myograph, DMT recommends that the myograph be weight-calibrated
at least once a month. The myograph should also be weight-calibrated every time the Interface has been moved.
Although lab benches are all supposedly perfectly horizontal, small differences in lab bench pitch can affect the
calibration of the system. The myograph also should be calibrated if the system has been idle for longer than a
month. A step-by-step procedure is included in the FORCE CALIBRATION sub-menu under SETTINGS, as explained in
“Chapter 3 - The Interface Menus”.
FORCE TRANSDUCER CALIBRATION PROCEDURE
This section contains step-by-step instructions to calibrate the force transducer.
CALIBRATION PROCEDURE IN CALIBRATED MODE
1. Move the mounting supports apart. Fill the chamber with distilled water or buffer. Use the same volume that will
be used during the experiments.
2. Set up the calibration kit (bridge and balance) on one of the myograph chambers as illustrated in
“Figure
4.1
- 4.2. Turn the heat on as discussed. The system takes about 20 to 30 minutes to reach 37°C. For skeletal
muscle preparations, most likely experiments will be performed at room temperature. It is recommended that
the system temperature be set to 25 to 27°C so that experiments are performed at a constant temper ature,
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NOTE: EVERY TIME A FORCE CALIBRATION IS PERFORMED THE MEASUREMENT RANGE
IS SET TO DEFAULT 200MN. SET MEASUREMENT RANGE AFTER THE FORCE CALIBRATION.
Summary of Contents for 820MO
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