DMT 820MO, User Manual, Page: 17 / 41

Step 1
involves setting up the chamber for calibration. Make sure
the chamber contains the pins or clamps, depending on the type of
tissue being studied. Fill the chamber with double-distilled water for
the volume to be used experimentally. Press
“NEXT STEP”.
Step 2
involves setting up the calibration kit appropriately for the
actual weight calibration. Verify that the transducer arm pin does
not touch the mounting pin for larger vessels or clamp for muscle,
as instructed. The calibration pin should be as close as possible to
the mounting pin or clamp without touching to get the most
accurate calibration. Press
“NEXT STEP” when the calibration kit
has been properly placed.
Step 3
initiates the heating process for the chambers. For the
calibration to be accurate, the transducers must be heated to the
experimental temperature to be used to accommodate heat-
induced expansion of the electronic parts in the transducer.
Otherwise, inaccurate readings and transducer drift may occur,
introducing large errors into the experiment.
.
17
CHAMBER 1 CALIBRATION
Step no.:
Turn the heat on. Wait until
the temperature is stable.
NEXT STEP BACK
3 1 2 4
6 5
Temperature setpoint: 37.0 °C
Probe temperature: 36.8 °C
HEAT ON HEAT OFF