CONTENTS
CHAPTER 1 - WIRE MYOGRAPH OVERVIEW
4
CHAPTER 2 - THE MULTI WIRE MYOGRAPH UNIT
5
2.1 Changing and adjusting the mounting supports
5
2.2 Calibration of the force transducer
8
CHAPTER 3 - EXPERIMENTAL SET-UP
9
3.1 Mounting protocol for small arteries
9
3.2 Mounting protocol for larger arteries
15
3.3 Normalization
17
3.4 Standard start
24
3.5 Endothelium function
25
3.6 In vitro experiment 1: Noradrenaline contractile response
26
3.7 In vitro experiment 2: Acetylcholine relaxation curve
28
CHAPTER 4 - CLEANING AND MAINTENANCE
30
4.1 Cleaning the 630MA Myograph system
30
4.2 Maintenance of the force transducer
32
4.3 Maintenance of the linear slides
36
APPENDIX 1 - BUFFER RECIPES
37
Physiological Saline Solution (PSS)
37
High potassium Physiological Saline Solution (KPSS)
39
APPENDIX 2 - NORMALIZATION THEORY
40
Mathematical calculations
40
APPENDIX 3 - READING A MILLIMETRE MICROMETER
42
