DIALAB DTN-410 K, User Manual

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DIALAB PHOTOMETER DTN-410K USER MANUAL
1- Introduction
1.1 General
The DIALAB DTN-410K is a semi-automated clinical chemistry analyzer, capable of storing
200 patients and up to 132 methods in memory, adequate for all routine tasks in Clinical and
Analytical Chemistry. A flow-through micro-cell with programmable aspiration volumes
enables sample load and sample recovery when required. Sample themosttating in cell is
achieved through a Peltier system with an operating range of 20 to 40º C. Additionally, a built-
in cell holder lodges standard or semi-micro cells for special purposes as coagulation or
turbidmetric measurements.
Versatile programming enables colorimetric, turbidmetric, endpoint and kinetic assays to be
performed in a simple way, referred to a standard, factor or calibration curve. Statistics can be
performed to controls for each stored method.
Bichromatic work mode is employed to reduce undesired side effects such as turbidity,
sample or reagent intrinsic color, presence of lint, dirt or small bubbles in flow-cell, etc., as it
accounts for all effects present at both wavelengths.
It is possible to perform analytical measurements using calibration curves obtained by
interpolation, with up to 10 standards or samples of known concentration.
Antibody dosage and other substances that require a cutoff value can be performed provided
there is a formula related to a negative and positive control.
The use of a calibration curve is recommended in all applications in which Lambert-Beer’s
Law does not hold, meaning a linear ratio between absorbance and concentration is not
attainable.
Concentration values for the construction of such a curve are entered through the keyboard.
The corresponding absorbances are read by the instrument or also keyed-in. This feature
enables the import of calibration curves defined in other instruments.
The optimum calibration curve is calculated once the standards have been measured or
entered. Various alarms and parameter displays inform operator of error in data entry. This
ensures user a reliable operation.
The possibility of replicating entries for standards and controls, for calculation of the
calibration curve or cutoff limit, ensures value verification.
This photometer admits the recovery of samples and measured standards, without
appreciable cross-contamination. Various readings can be averaged for accuracy enhance.
Calibration curves and readings can be performed in endpoint or bichromatic endpoint modes.
Samples and standards measured in this way are the result of reading at one wavelength, or
the difference between absorbance readings at two wavelengths, or the slope of a kinetic
process.