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PAGE 7
DIAGENODE
BIORUPTOR
®
PLUS
USER MANUAL
Diagenode Inc. North America
/ Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na
@
diagenode.com
Getting to Know Your Bioruptor
®
Plus
System
Bioruptor
®
Components Overview
Sonication bath
The sonication bath is a critical component of the instrument. The generators below the tank
produce ultrasonic waves which are then transferred through water. The sonication bath
requires special handling and care as described below.
Handling
The sonication bath must remain upright at all times, especially when moved. Tilting the
sonication bath or handling roughly may damage the ultrasound emitter component,
resulting in a substantial drop in sonication efficiency. If transportation of the apparatus is
required after initial set-up, it is imperative to keep the tank at a right angle to the ground during the transport at all
times.
Water level and quality
The level of the water has been optimized and should always reach the red line (sticker on the wall of the tank). Use only
distilled water to fill the tank. Do not use deionized water. Replacement stickers can be obtained from Diagenode.
Water temperature
The water in the sonication bath must be kept at
4°C
. Ultrasonic waves produced by the Bioruptor
®
generate heat. Drop
off in efficiency will occur above 10°C. To ensure preservation of the samples and to prevent damage to the instrument,
it is necessary to start the sonication process with cold water and to keep it at 4°C during the sonication process.
Power Cable
Control Unit
Sonication bath
Motorized lid
Soundproof Box
Control Unit Cable
Tube holder example
Power Supply Adaptor
(only outside the U.S.)