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DIAGENODE
BIORUPTOR
®
PLUS
USER MANUAL
Europe Diagenode sa
/ CHU - Tour GIGA - B34 - 3e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info
@
diagenode.com
Critical Steps for Maintenance and
Efficient Shearing
General warnings
•
DO NOT
turn on the instrument without water.
•
DO NOT
tilt the sonication bath. To change the water, use either the plastic pump or a
beaker.
Water level (sonication bath)
• The sonication bath must be filled with distilled water only to the fill line (do not use deionized water). Fill line
replacement stickers can be obtained by contacting Diagenode. Change water at least once per week.
Water temperature (sonication bath)
• Optimal water temperature for sonication is 4°C. Sample temperature should not exceed 10°C.
• Methods to maintain the temperature.
- Ice - add small amounts of crushed ice (no more than 0.5 cm) to the bath every 10 minutes.
-
Water Cooler (Cat. No. BioAcc-Cool) - the flow rate of the circulating water cooler cannot exceed 500 ml per
minute for optimal sonication.
Magnetic Ultrasound Emitter Maintenance
• The ultrasound waves are created from a series of magnets that are attached to the water tank. This system is very
sensitive to the heat generated during a run.
• Do not exceed 1 hour of total sonication per run. It is critical that the machine is allowed to cool at least 20 minutes
between runs. Damage resulting from non compliance to manual instructions will void the warranty and shorten the
lifespan of the machine.
• Ultrasound Emitters can be damaged by tilting or jarring the machine. Exercise care if moving water tank.
Validated tubes for the Bioruptor
®
Plus
• DNA shearing: 0.5 ml Bioruptor
®
Microtubes for DNA Shearing (Cat. No. WA-004-0500).
• Chromatin Shearing: 1.5 ml TPX microtubes (Cat. No. M-50050 or M-50001) and 15 ml TPX tubes (Cat. No. M-UN-15).
Others tubes might be used but will require additional optimization. Once a brand of tube is optimized, switching brands
may result in changes in sonication efficiency.
Fitting 0.5 ml or 1.5 ml tubes in the corresponding tube holder
1. Place the tubes on the corresponding tube holder (0.5/0.65 ml tube holder - Cat. No. UCD-pack 0.5 or 1.5 ml tube
holder - Cat. No. UCD-pack1.5). Never leave empty spaces in the tube holder. Fill the empty spaces with tubes
containing the same volume of water. Screw the lid on the tube holder without over-tightening the lid.
2. Carefully place the tube holder on the holding plate.
3. During sonication, samples must remain at the bottom of the tube. If needed, briefly centrifuge samples during
sonication after pausing the run.
Fitting of 15 or 50 ml tubes in the corresponding tube holder
1. Loosen both the blue and the black top prior to placing the metallic reflecting bar in the tube.
2. First tighten the blue ring then the black top. This will ensure the O-ring is properly placed in the tube.
Biorup
tor
