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DIAGENODE
BIORUPTOR
®
PLUS
USER MANUAL
Diagenode Inc. North America
/ Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na
@
diagenode.com
Samples are vortexed (10-15 sec) and centrifuged (10 sec) before shearing.
For optimal results samples should be stored on ice during 10-15 minutes prior to sonication.
Temperature:
Maintain at 4°C by using ice chilled water and small amounts of crushed ice (no more than 0,5 cm)
or use the Bioruptor
®
Water Cooler (Cat. No. BioAcc-Cool).
Power setting:
L position (Low)
Sonication cycle and sonication time:
varies depending on desired DNA size (see table)
Note:
Recommended protocols are subject to change without notice. Additional protocols are available on demand.
Target size
Cycle conditions (On/Off times in sec.)
Number of cycles
1250 bp
15/90
2 cycles
950 bp
15/90
4 cycles
750 bp
30/90
3 cycles
550 bp
30/90
5 cycles
400 bp
30/90
6 cycles
350 bp
30/90
8 cycles
300 bp
30/90
10 cycles
250 bp
30/90
15 cycles
200 bp
30/90
30 cycles
150 bp
30/30
70 cycles
*recommended to use a lab timer to set time precisely
The protocol settings listed above are recommended guidelines and actual results may vary depending on the type and amount
of starting material, purity level, concentration and/or sample viscosity. It is highly recommended that a time course response
experiment be carried out (e.g. varying the time of “on” and “off” durations as well as the number of cycles) to determine the
appropriate treatment for your specific sample. Starting material with a smaller sample volume and a greater concentration
than the recommended range may require a different time course to ensure homogenous shearing results.
