5.2
Pack the column
Introduction
This section describes how to pack the column and the tools and reagents required.
Note:
It is important to read all of the information in this chapter before using the
column!
Items required for packing
To pack the column, the following items are needed, in addition to the Primer Support:
Item
Quantity
Product
code
Adjustable Oligo Column
1
18110760
Tubing Connectors for OD 1/8" tubing
For the column side M6 connector
For the system side
2 of each
18117264
18112117
Ferrules for OD 1/8" tubing
4
18112118
Tubing
1
ID 1.6 mm OD 1/8"
2
18112163
1
Cut the tubing to a suitable length.
Calculate column volume
From the table below, calculate the column volume (CV) for the dry Primer Support
corresponding to the intended synthesis scale. The values in the table correspond to
use of Primer Support 5G.
(g) (mL)
(mL)
2.5
23
5
45
10
90
15
135
22
198
Note:
The Primer Support must be packed as a slurry.
The exact ratio is 0.111 gram Primer Support 5G per mL column volume.
Calculation to determine the CV:
3 × 3 × 3.14 × bed height in cm = CV in mL
5 Operation
5.2 Pack the column
22
Adjustable Oligo Column Operating Instructions 28967444 AC
