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Adjustable Oligo Column

Operating Instructions

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Summary of Contents for 18110760

Page 1: ...Adjustable Oligo Column Operating Instructions cytiva com...

Page 2: ...tion 21 5 2 Packthecolumn 22 5 3 Unpackandemptythecolumn 24 6 Maintenance 25 6 1 Disassembleandassembleforcleaningandmaintenance 26 6 2 Cleaning 27 6 3 Partreplacement 28 7 Troubleshooting 30 8 Refere...

Page 3: ...Pageintentionallyleftblank...

Page 4: ...formation intendeduseof AdjustableOligo Column andlistsofabbreviationsusedinthisdocumentation Inthischapter Section Seepage 1 1 Importantuserinformation 6 1 2 Aboutthismanual 7 1 3 Abbreviations 8 1 I...

Page 5: ...leusingPrimerSupport Thecolumnisdesignedfor maximumscalabilityandcouplingefficiencywithaflexiblecolumnvolume AdjustableOligoColumn isintendedforusewith KTA OligoPilot plus100 AdjustableOligoColumn mus...

Page 6: ...desinformationneededtoinstall operateand maintaintheproductinasafeway Scopeofthismanual Thismanualdescribeshowtouse AdjustableOligoColumn Theillustrationbelow shows AdjustableOligoColumn 1 Introductio...

Page 7: ...iations Abbreviation Meaning CV Columnvolume EPDM Ethylenepropylenediene monomer ETFE Ethylene tetrafluoroethylene ID Internaldiameter OD Outerdiameter PEEK Polyetheretherketone PTFE Polytetrafluoroet...

Page 8: ...ent Inthischapter Section Seepage 2 1 Safetyprecautions 10 2 2 Symbolsandabbreviations 14 Important WARNING Beforeinstalling operatingormaintainingtheproduct all usersmustreadandunderstandtheentirecon...

Page 9: ...ion Maintenance Definitions Thisuserdocumentationcontainssafetynotices WARNING CAUTION andNOTICE concerningthesafeuseoftheproduct Seedefinitionsbelow WARNING WARNINGindicatesahazardoussituationwhich i...

Page 10: ...as sufferedanydamage forexampleifdamageiscausedbydropping theequipment Personalprotection WARNING Hazardoussubstancesandbiologicalagents Whenusing hazardouschemicalandbiologicalagents takeallsuitable...

Page 11: ...thesuppliedspannerorwrench DonotovertightenPTFEtubingasthiswilldamagetheendofthe tubing WARNING Neverusepressurelimitsabovethecolumnspecification WARNING Neverexceedtheoperatinglimitsstatedinthisdocum...

Page 12: ...ggedastakenoutofoperation allprocesswettedareasarecleananddecontaminated WARNING Decontaminatebeforemaintenance Toavoidpersonnelbeing exposedtopotentiallyhazardoussubstances makesurethatthe columnispr...

Page 13: ...ion Descriptionofsymbolsonthecolumn label Thefollowingsymbolsmaybepresentonthecolumnlabel Label Meaning FollowinstructionsforuseintheOperating Instructions manual Warning Readtheuserdocumentationbefor...

Page 14: ...ods Note If the support rods between the two end pieces have been removed and refitted they must be tightened with a torque key to 1 45 to 1 55 Nm for safe operation Thebottomend piececontainsthebotto...

Page 15: ...8 6 9 10 11 2 Part Function 1 Securingringadapter 2 Connectionpointtothecolumn 3 PrefilterunitincludingO ring 4 TubingD flange 5 O ring 64 5 3mmdiameter 3 Productdescription 16 AdjustableOligoColumnO...

Page 16: ...ion 6 O ring 55 1 6mmdiameter 7 Glasscolumntube 8 Distributionunit 9 Securingring 10 Connectionpointfromthecolumn 11 Tierodandhex nut 3 Productdescription AdjustableOligoColumnOperatingInstructions289...

Page 17: ...d with the adapter fitted in the column Unpacking Ondeliveryofthecolumn performthefollowingsteps Step Action 1 Opentheboxandcarefullyremovethecolumn 2 Inspectthecolumnforanydamage 3 Reportanydamagetoy...

Page 18: ...iallydangerousifhazardouschemicalsareinuse Connectthetubingbyfirstinsertingthetubingfully then tighteningtheconnectorfingertight PEEKtubingshouldbe tightenedafurther1 4turnusingthesuppliedspannerorwre...

Page 19: ...NOTICE Toavoidprecipitationkeepallreagentsatorabove18 C 5 Operation 20 AdjustableOligoColumnOperatingInstructions28967444AC...

Page 20: ...Downflow Canhelpsomesupportstospreadthe reagentsmoreevenly Reagentsenterthe columnthroughthebottomfilterandexit throughthetopadapterfilter Usingflowdirectionfrombottomtotop alsosimplifieschangingadir...

Page 21: ...hesystemside 2ofeach 18117264 18112117 FerrulesforOD1 8 tubing 4 18112118 Tubing1 ID1 6mmOD1 8 2 18112163 1 Cutthetubingtoasuitablelength Calculatecolumnvolume Fromthetablebelow calculatethecolumnvolu...

Page 22: ...on 6 Adjustthecolumnadaptertothelevelthatcorrespondstothecalculated volumefromthelistinCalculate column volume on page 22 7 ConnectthesystemtotheinletofthecolumnusingaPTFEtubewithanM6 connector 181172...

Page 23: ...mntoallow theacetonitriletocomeoutatthebottomofthecolumn 4 ContinuetheairflowuntilthePrimerSupporthasreducedinvolumeandis freefromthecolumnwall Note This will take approximately 30 minutes depending o...

Page 24: ...y The AdjustableOligoColumn isdesignedwithaminimumofcomponentstofacilitate cleaningandoperation Inthischapter Section Seepage 6 1 Disassembleandassembleforcleaningandmaintenance 26 6 2 Cleaning 27 6 3...

Page 25: ...lockwiseby handandpullitoutofthecolumnbody Assembly Toassemble AdjustableOligoColumn followthesesteps Step Action 1 Findtheguidescrewontheinsideofthecolumnbodyandthegrooveonthe outsideoftheadapter 2 I...

Page 26: ...nbeforeperformingany maintenanceactivities service orreturningtheproduct Cleanthefrits Pumpmethanolslowlyfor30minutesthenleavetoletdry Note Do not sonicate the frits Testthecleaneddistributionplates I...

Page 27: ...oppieceandtheblacksecuringO ring Lift theadapterpre filterhousingfree 4 Usetheplasticwrenchtodisconnectthelengthoftubingfromthepre filter housingthatconnectsthepre filterhousingtothedistributionunitat...

Page 28: ...O ringreplacement TwodifferenttypesofO ringsareusedinthe AdjustableOligoColumn Useaspatula topryoutwornO rings 6 Maintenance 6 3 Partreplacement AdjustableOligoColumnOperatingInstructions28967444AC 29...

Page 29: ...alblockage Cleanallvalvesand tubingandremoveany internalblockage Columnfiltersare blocked Airmaybe trappedinthefilter Alwayswetandflushwith 95 ethanolor acetonitrilebeforeuse Tubingisbent Straightenth...

Page 30: ...ation regulatory information orderinginformation andHealthandSafetyDeclarationformsforservice Inthischapter Section Seepage 8 1 Specification 32 8 2 Chemicalresistance 33 8 3 Recyclinginformation 35 8...

Page 31: ...m 1 Thedesignpressureisthesumofpackingandoperatingpressures Thedesignpressuremust notbeexceeded Note Using Primer Support 5G according to recommended packing procedures adds no packing pressure Dimens...

Page 32: ...swettedpartsaremanufacturedfrom fluoroplastic borosilicateglass polypropylene polyethylene ETFE titanium Chemicals Theinformationinthetablebelowhasbeencompiledfrompublishedmaterialfrom severalsources...

Page 33: ...tration Sodiumhydroxide 2M Trifluoroacetic acid 0 1 NOTICE DonotuseTHF tetrahydrofurane in AdjustableOligoColumn 8 Referenceinformation 8 2 Chemicalresistance 34 AdjustableOligoColumnOperatingInstruct...

Page 34: ...theproduct Whentakingtheproductoutofservice thedifferentmaterialsmustbeseparatedand recycledaccordingtonationalandlocalenvironmentalregulations Recyclingofhazardoussubstances Theproductcontainshazardo...

Page 35: ...troubleshootingreports visit cytiva com contact Manufacturinginformation Thetablebelowsummarizestherequiredmanufacturinginformation Requirement Information Nameandaddressofmanufacturer CytivaSwedenAB...

Page 36: ...gadapter 1 18111572 Glasstube 1 18111533 Distributionunit 1 29042220 Securingring60 1 18111573 TierodincludingHex nut 1 18111534 ETFEtubingID1 6mm OD1 8 3m 1 18112116 Tubingtoadjustablecolumn 1 181115...

Page 37: ...e cases this may require customer to move equipment from normal operating location prior to Cytiva arrival Consumables such as columns or gels have been removed or isolated from the instrument and fro...

Page 38: ...t be decontaminatedprior to service return Provide a telephone number where Cytiva can contact you for additional information concerning the system equipment Telephone No Liquid and or gas in equipmen...

Page 39: ...ion 36 N Notesandtips 6 O O ringreplacement 29 Onsiteservice 38 P Packcolumn 22 23 calculations 22 procedure 23 tools 22 Prerequisites 6 Productreturnorservicing 39 Purposeofthismanual 7 R Recyclingin...

Page 40: ...a Anyuseof UNICORN issubjectto Cytiva StandardSoftwareEnd UserLicenseAgreementforLifeSciencesSoftwareProducts Acopy ofthisStandardSoftwareEnd UserLicenseAgreementisavailableonrequest Allgoodsandservic...

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