CURIOSIS Celloger Mini Instruction Manual Download Page 1

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Automated live cell imaging system

Celloger Mini

Instruction manual

Summary of Contents for Celloger Mini

Page 1: ...www curiosis com Automated live cell imaging system Celloger Mini Instruction manual ...

Page 2: ...IOSIS Inc Email info curiosis com Website www curiosis com CURIOSIS Inc 4F 10 Teheran ro 38 gil Gangnam gu Seoul 06221 South Korea Tel 82 2 508 5237 Fax 82 2 508 5246 Copyright 2021 by CURIOSIS Inc All rights reserved Published in Korea ...

Page 3: ...n mode File storage and project name Time setup 3 View image file Album mode 4 Movie maker Album mode Image analysis Analysis mode 1 Confluency 2 Growth curve Safety information General guidelines Operating condition Safety standards Cleaning and Maintenance Trouble shooting Product specifications Ordering information Appendix A Connecting multiple devices Appendix B How to insert Vessel or Vessel...

Page 4: ... Celloger App 1 Vessel holder for dish 60mm Optional 1 Vessel holder for dish 35mm Optional 1 Vessel holder for T flask 25cm2 Optional 1 When receiving the package Check that all items listed above are included in your package Examine the device carefully for any damage during shipping Contact your local distributor or info curiosis com if any items are missing or damaged Any loss or damage claims...

Page 5: ...5 Device layout ...

Page 6: ...vice from the box ensure to hold the bottom of the device Brightfield lamp is located at the center of the device and it is not a handle 2 Take off the lens cover black 3 1 Connect to power cable Previous version A Plug the power cable into device and connect to PoE P DATA OUT Right side ...

Page 7: ...ect the device to PC using the connection cable and PoE DATA IN Left side 3 2 Connect to power cable Current version A Plug the power cable into PoE B Connect the device to PC using the connection cable and PoE ...

Page 8: ...nu 7 Click the Ethernet button and right click the mouse and click Properties in the window that appears 8 Select Internet Protocol Version 4 TCP IPv4 and click Properties 9 Select Use the following IP address and input IP address and Subnet mask Note IP address should be 192 168 2 and in field input 2 254 except 10 For Subnet mask input 255 255 255 0 The PC IP address should be set differently fr...

Page 9: ...9 ...

Page 10: ...ral time lapse imaging High 2592 x 1942 pixel resolution Used for a single capture Able to get a clearer image than Normal resolution Caution To obtain the confluency data you must capture or scan images using Normal High does not perform analysis it is only possible to analysis an image that is Normal resolution Lamp Meaning that currently lamp is brightfield Objective Lens Meaning that lens magn...

Page 11: ...me lapse images X Y Z position It shows the current coordinates of X Y Z Imaging control panel AutoFocus Execute autofocusing depending on the autofocus settings in streaming status refer to page 18 for autofocus setting Preview Start Streaming Preview on Stop Streaming Preview off Temperature Humidity Thermometer hygrometer next to the LED light source in the bright field Note The temperature and...

Page 12: ...pter Connection status Showing device connection status Device IP address IP address of connected device Device version Firmware version of connected device 17 Display It shows live or captured images Mode setting panel Section changes depending on mode Device IP address Device version Connection status ...

Page 13: ... in Connect Device and click OK and then the device is connected Note If the device icon is not shown on the window please check the followings Check the PC IP address setting page 8 In case of multiple devices refer to APPx A page 30 When Adapter is connected select Network Adapter and select connected adapter by pressing an arrow ...

Page 14: ...t the vessel type in Setup mode Well plate dish flask can be selected Upon selecting vessel type the vessel type is displayed on the plate panel at the bottom right Note A vessel adapter suitable for vessel type must be used use vessel adapter for vessels except for well plate 4 Click and move desired vessel location on the plate panel with the mouse Upon clicking vessel or well in Plate panel it ...

Page 15: ...op the movement of stage It is possible to adjust motor speed and step distance Speed control On the left circular slider move the green arrow to change the value at the center of the circle can select the value from 10 to 100 Step control On the right circular slider move the blue arrow to change the value at the center and adjust the unit after opening combo box alternative keyboard 1 um 10 mm N...

Page 16: ...m 0 to 160 Note The recommended values are as follows Lamp power 135 Brightness 256 Contrast 40 The higher the difference between cell and background the better calculation of confluency page 33 9 Select Image Format and Frame Resolution in Imaging Frame Resolution can also be selected on the left side of the display 10 To save the image right click on the display and click Save As The saved image...

Page 17: ... In the AF panel in Setup mode autofocusing can be set Depth mm Range of AutoFocus fuction Resolution mm Scanning unit within depth ROI Section The area can be selected and AutoFocus algorithm is executed based on the selected area Plate panel Possible to set scanning order by right clicking the mouse on Plate panel The table on the left side of the Plate panel shows Scan Position Set Move to cent...

Page 18: ...r autofocusing is run on Time lapse imaging or not Able to delete all scan positions designated To delete just one scan position select the location to be deleted and press Delete key on the keyboard Import the exported scan position table Save scan position table Features on display Display features of the Display screen Measure confluency mark using icon located at the right bottom of Display Me...

Page 19: ...e As Save images raw image and captured image with features are saved Stage Position Show current position on the right top of Display Scale Bar Show scale bar on the left bottom of Display Histogram Display Intensity Histogram of images Center Mark Display the reference point at the center of display in cross mark Video Area Area shown in the video upon making video files Grid Lines Draw grid lin...

Page 20: ...Scan position set Note Note If Auto Focus is checked in and Set Position is executed autofocusing function is executed automatically Align Image is location correction function It corrects location if the location is stepped out compared with the previous cycle during scanning However if interval time is 6 hours or longer or changes in morphology are extreme within a short period of time differenc...

Page 21: ...Scan progress Cycle Scanned set position total set position Cycle Time Remaining Time remaining until the start of the next cycle Elapsed Time Time since the scanning starts Start Date Date and time of starting scanning End Date Date and time of ending scanning Important Input the equipment and plate into an incubator for more than 30 minutes for warming up to prevent condensation before starting ...

Page 22: ...evel folder shows the images in the selected folder Note To make a video file data should be obtained from CellogerMiniApp 3 Adjust Crop ratio and FPS Crop ratio means video area and FPS means frame per second Note It is recommended to set Crop ratio at 10 or higher 4 Press Play button to check time lapse images that are displayed consecutively from the point when the button is pressed Upon pressi...

Page 23: ...image from images saved in a computer Time Lapse Images Select a desired file among files scanned in CellogerMiniApp 3 Adjust parameters in Color Note The alpha button controls the transparency or opacity of a color 4 Conduct confluency analysis by pressing Run 5 The green part below is confluency and the area of cells analyzed is shown as cell mark To turn off the cell mark in press for cancellat...

Page 24: ...ut cell mark does not follow 2 Growth curve 1 Select Analysis mode 2 On Source select Time Lapse Images and open the file from which you want to get the result 3 Control parameters on Color 4 Press Run to obtain confluency It is possible to show confluency in Data every cycle and obtain growth curve on Graph ...

Page 25: ...e to boot Do not insert any metallic objects into the device through bottom and side air vent to avoid electrical shock causing personal injury or device damage Place the device around 10cm away from the surroundings for proper air cooling Do not disassemble the device in any event Contact your local distributor to arrange for service in case of malfunctioning Operate the device carefully as descr...

Page 26: ...it first in a dry and well ventilated place and store it As the device uses X Y Z axis motor it is sensitive to vibration and horizontality It should be used in a flat place without vibration Never disassemble the instrument yourself Do not remove any covers or parts that require the use of a tool to obtain access to moving parts Operators must be trained before being allowed to perform the hazard...

Page 27: ...wab Eliminate any dust on culture dish and LED lamp Remove any condensation on the lid of the culture dish Problem in saving files Check the unused volume of the hard disk Time lapse images become dark and bright Warm up the instrument 30 minutes before monitoring Eliminate dust on culture dish Check for and remove any condensation on the lid of the culture dish In order to prevent condensation fr...

Page 28: ...evice Refer to recommended minimum interval time page 21 If the interval time is long and align image is set align image correction function may not work properly Reduce interval time or cancel align image setting page 21 Others The values in Temperature Humidity of Celloger Mini are different from those of incubator or separately installed thermometer and hydrometer The temperature and humidity s...

Page 29: ...er requirements 100 240V 50 60Hz Output ports Ethernet Computer External PC O S required Window 10 Processor recommended CPU 3G Storage recommended 1TB Monitor Recommended 1920 x 1080 Accessories Vessel holders T flask A25 Petri dish 35mm Petri dish 60mm PoE adapter ethernet cable USB memory Warranty 1 year Ordering information Catalog No Product Name Description CRCLG MB01 CELLOGER Mini Live cell...

Page 30: ...ltiple devices with the same IP address is connected to a PC and only one icon appears on Connect Device change the IP address of devices except for one device Make sure that the same IP address of multiple devices is not entered and device IP address is set differently from PC IP address 1 Select Device icon and double click the Device IP address at the right bottom of the screen to open Device S...

Page 31: ...evice IP address is lost there is no way to know the IP address In case of changing address changed IP address should be written down on a separate sheet 4 Turn off the device and then turn it on again 5 Run CellogerMiniApp to check whether IP address has changed Note The device currently connected and running can be identified by clicking or checking the Device IP address at the bottom of the scr...

Page 32: ...e insert the area where it contacts with a ball plunger and insert it on the other side Turn the knob upon inserting vessel into a vessel adapter or removing it from a vessel adapter Turn Turn Ball plunger located inside Area where it contacts with a ball plunger Adapter or Well plate Picture of inserting an adapter in a tilted state Picture showing complete mounting of an adapter ...

Page 33: ...ocus Most of the cells look clearly Raw image Image with cell mark 2 Out of focus Some cells are difficult to see as they are separated from the background and are not visible Raw image Image with cell mark 3 Wrong light intensity selection 4 Correct light intensity selection ...

Page 34: ...ro 38 gil Gangnam gu Seoul 06221 South Korea TEL 82 2 508 5236 FAX 82 2 508 5246 Email info curiosis com FACTORY 400 Wonam ro Namsa myeon Cheoin gu Yongin si Gyeonggi do 17123 South Korea TEL 82 31 339 6404 FAX 82 31 339 6409 www curiosis com ...

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