20
Time-lapse imaging
1.
Scan position setup (Setup mode)
1.
Place the device into an incubator.
2.
Mount vessel when the stages comes to the front after pressing . (Install vessel after installing vessel
adapter first in case of dish or flask.)
3.
Select Vessel type and desired location on the plate panel.
4.
Press
Start Streaming
.
5.
Move to the desired location in the
Setup
mode.
(page 15 step 6)
6.
Adjust focus with
Jog
or
Autofocus
.
(page 17)
7.
Adjust
brightness
,
contrast
or
lamp power
, if necessary.
(page 16 step 8)
8.
Select
Image Format
and
Frame Resolution
on
Imaging
.
9.
Designate the position by pressing
on desired location. After designation, designated location is shown
on the plate panel and scan position set (
page 17
for more details about Plate panel and Scan position set).
Note
: Note : If Auto Focus is checked in
and Set Position is executed, autofocusing function is
executed automatically. Align Image is location correction function. It corrects location if the location is stepped
out compared with the previous cycle during scanning. However, if interval time is 6 hours or longer or changes in
morphology are extreme within a short period of time, differences in image from previous cycle becomes large,
having a negative impact so in this case, it is better to turn off the setting.
2. Scanning (Scan mode)
Designation of file storage location and project name
In Scan mode, set the location (location where files will be saved) and project name. In the Location file, folder is
created in the name of project name and date set. If location designation is skipped, the file is saved in the scan file
and project name is not designated, folder name is created with the start time. Folder is created for each Set
Position.
