ChemoMetec NucleoCounter, User Manual

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1 Inspection and Unpacking of Equipment
1
Table of contents
DECLARATION OF CONFORMITY ....................................................................... VI
WEEE DIRECTIVE INFORMATION
– EUROPE ONLY ......................................... VIII
INTRODUCTION AND INTENDED USE ................................................................. X
WARNINGS AND PRECAUTIONS ....................................................................... XIII
1 INSPECTION AND UNPACKING OF EQUIPMENT ........................................... 3
2 INTRODUCING THE NUCLEOCOUNTER ........................................................ 5
2.1 T
HE
N
UCLEO
C
OUNTER INSTRUMENT
.............................................................. 5
2.1.1 Fluorescence microscope ............................. 6
2.1.2 Built-in image analysis ............................. 7
2.2 T
HE
N
UCLEO
C
ASSETTE
............................................................................... 8
2.2.1 Loading the cassette ................................ 8
2.2.2 Fluorescent dye ..................................... 8
2.2.3 Sample volume ....................................... 9
2.2.4 Cassette handling ................................... 9
2.3 L
YSIS BUFFER
(R
EAGENT
A100)
AND
S
TABILIZING BUFFER
(R
EAGENT
B) ........... 9
3 CONTROL BUTTONS, KEYPAD AND DISPLAY .............................................. 12
3.1 I
NTERACTIVE
C
ONTROLS
............................................................................. 12
3.1.1 Control Buttons .................................... 12
3.1.2 Keypad ............................................. 13
3.2 I
NTERFACE
D
ISPLAY
................................................................................... 13
3.2.1 Status indicator ................................... 13
3.2.2 Display screen ..................................... 13
4 INSTALLATION AND START-UP .................................................................... 14
4.1 P
OW ER ON
,
POWER OFF
.............................................................................. 14
4.1.1 Starting up ........................................ 14
4.1.2 Shutting down ...................................... 15
4.2 I
NSTALLATION OF
N
UCLEO
V
IEW
................................................................... 15
5 OPERATION OF THE NUCLEOCOUNTER ...................................................... 16
5.1 R
EADY MODE
............................................................................................. 16
5.2 I
NSERTING AND REMOVING THE CASSETTE
..................................................... 16
5.2.1 Protection of the optical system ................... 16
5.2.2 Inserting cassette ................................. 16
5.3 M
EASURING
- T
HE
R
UN BUTTON
................................................................... 17
5.3.1 Actuator movements ................................. 17
5.3.2 Result presentation ................................ 18
5.3.3 External computer .................................. 18
5.4 R
ESULT DISPLAY
........................................................................................ 18
5.4.1 Result mode ........................................ 18
5.4.2 Saving parameters .................................. 19