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Cantium Scientific MicroBio MB2, Operating Manual

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Cantium Scientific MicroBio MB2 Operating Manual Download Page 14

Sampling

The selection of the sample volume is important for reliable sampling.  If the 
contact plate or petri dish is overloaded with colonies it is difficult to make an 

accurate  count.  With  experience,  the  user  will  anticipate  the  probable 
bioaerosol  concentration  in  an  area,  but  it  may  be  necessary  to  make  a 
preliminary survey at a number of sampling volumes to identify the optimum 
setting.  Each  sample  should  be  repeated  several  times  and  a  statistical 
mean value and confidence value determined.

Selection of Media

The agar media used in the contact plate or petri dish should be chosen to 
suit  the  micro-organisms  being  monitored.  For  a  wide  range  of  micro-
organisms, consider using tryptone soy agar (TSA), casein soy peptone agar 

(CPSA)  or  nutrient  agar  (NA).  There  are  other  selective  agars  for  more 
specific micro-organisms. For fungi (yeasts and moulds), consider using malt 
extract agar (MEA) or rose bengal agar (RBA).   

Appendix C

 details various 

culture media types.




IQ / OQ / PQ

Documentation  templates  are  available  from  Cantium  Scientific  Limited  or 
your  local  distributor  to  support  in-house  Installation,  Operational  and 
Performance Qualification. 

13

Summary of Contents for MicroBio MB2

Page 1: ...MicroBio MB2 MB2 HiFlow and MB2 RSH Bioaerosol Sampler Operating Manual ...

Page 2: ...ttribution Non Commercial ShareAlike 4 0 International License https creativecommons org licenses by nc sa 4 0 Qualisair is a registered trademark of Cantium Scientific Limited Cantium Scientific Limited Clarendon Gardens Dartford Kent DA2 6EY United Kingdom Tel 44 0 1322 252000 www cantiumscientific com October 2019 Issue 5 Printed in England 1 ...

Page 3: ...ing Battery 11 Battery Management 12 Sampling 13 Selection of Media 13 IQ OQ PQ 13 Inserting a Contact Plate or Petri Dish 14 Switching On and Off 15 Take a Sample 16 Delayed Sample 18 Sequential Sampling 19 Sequential Sampling How it works 19 Sequential Sampling Configuration 20 User Presets 23 Unit Status 24 Battery Level 24 Power Diagnostics 24 Total Volume Count 25 Total Samples Taken 25 2 ...

Page 4: ...alibration 33 Troubleshooting and Technical Support 34 Appendix A 220 Hole Count Correction Table 35 Appendix B 400 Hole Count Correction Table 36 Appendix C Culture Media Types 37 Appendix D Replacement Parts 38 Appendix E Bioaerosols 39 What is a bioaerosol 39 Sources of Bioaerosols 39 Monitoring of Bioaerosols 40 Appendix F MB2 RSH Installation 41 Fitting the Connector for Unterminated Cables 4...

Page 5: ...ibility EMC Directive 2014 30 EU BS EN 61326 1 2013 Scientific Test and Measurement Equipment Referencing BS EN 55011 CISPR11 Emissions for Industrial Scientific and Medical Equipment BS EN 61000 4 2 Immunity to Electrostatic Discharge BS EN 61000 4 3 Radiated Immunity Restriction of the Use of Certain Hazardous Substances RoHS in Electrical and Electronic Equipment EEE Directive 2011 65 EU BS EN ...

Page 6: ...roBio MB2 key parts are manufactured from the following These items must be taken to approved recycling centres equipped to handle such waste and recover the metals used in their manufacture Some elements of these parts cannot be recycled Enclosure Material Recyclable Blower housing Aluminium Yes Air blower Glass reinforced nylon Yes limited facilities Enclosure ABS Plastic Yes Display window Poly...

Page 7: ...t or if necessary a replacement This warranty does not cover accidental damage wear and tear consequential or incidental loss The warranty excludes rechargeable cells supplied with the sampler Damage caused by cleaning materials and methods not recommended by the manufacturer use beyond the specification use in wash down areas unless used in approved protective bags or modifications without prior ...

Page 8: ...es Flow Rate 100 L min 1 Sample Volume 10 to 10 000 litres in varying steps Sampling Volume Capacity 60 000 litres before recharge d50 Particle size 1 7 µm 220 x 1mm hole head 1 35 µm 400 x 0 7mm hole head Mean particle velocity 9 62 ms 1 220 x 1mm hole head 10 7 ms 1 400 x 0 7mm hole head Weight excluding charger and carry bag 780 g inc battery and petri dish Dimensions 196 x 100 x 110mm inc head...

Page 9: ...013mbar 20ºC Environmental conditions will affect air pressure thus mass volumetric flow rates Flow Rate 180 L min 1 Sample Volume 10 to 10 000 litres in varying steps Sampling Volume Capacity 40 000 litres before recharge d50 Particle size 1 71 µm Mean particle velocity 9 55 ms 1 Weight excluding charger and carry bag 840g inc battery and petri dish Dimensions 196 x 100 x 110mm inc head Power 4 x...

Page 10: ...Sample Volume 10 to 10 000 litres in varying steps Sampling Volume Capacity 60 000 litres before recharge d50 Particle size 1 7 µm 220 x 1mm hole head 1 35 µm 400 x 0 7mm hole head Mean particle velocity 9 62 ms 1 220 x 1mm hole head 10 7 ms 1 400 x 0 7mm hole head Weight excluding charger and carry bag 1680 g inc controller head unit battery cable petri dish Dimensions 196 x 100 x 40mm control un...

Page 11: ...Department of Trade and Industry Validation of Analytical Methods VAM programme The sampler collects airborne micro organisms by drawing a stream of air at a constant flow rate through a series of small holes in a metal head Particles suspended in the air stream impinge onto the surface of a sterile culture medium in a contact plate or petri dish After exposure to a set volume of air the contact p...

Page 12: ...r the serial number label and then slide downwards The orientation of the cells is indicated within the battery compartment Carefully replace the lid with an upwards sliding motion ensuring the OPEN marking on the lid is towards the serial number label NOTE Please read the instructions supplied with the charger before charging the NiMh cells To avoid corrosion we recommend cells are removed from t...

Page 13: ...h quality such as Duracell Energizer or Varta industrial range If using alkaline cells the battery type needs to be manually set Please see the Settings section of this manual for instructions As cells age through time and use their internal electrical resistance impedance increases This will reduce the amount of time the sampler can be used on a single charge Old cells may at random when under lo...

Page 14: ... confidence value determined Selection of Media The agar media used in the contact plate or petri dish should be chosen to suit the micro organisms being monitored For a wide range of micro organisms consider using tryptone soy agar TSA casein soy peptone agar CPSA or nutrient agar NA There are other selective agars for more specific micro organisms For fungi yeasts and moulds consider using malt ...

Page 15: ...y on the support plate then re fit the sampling head The standard MicroBio MB2 can use 220 or 400 hole sampling heads The 220 hole sampling head can be used with both 55mm 65mm contact plates or 90mm petri dishes but only the centre 55mm of the petri dish will collect samples Using the 400 x 0 7mm hole sampling head will give nearly full coverage of a 90mm petri dish improving reliability of count...

Page 16: ...k and not allow samples to be taken that may not complete To switch off the MicroBio MB2 press the ON OFF button The MicroBio MB2 will automatically switch off after a period of inactivity Automatic and manual switch off is prevented during sampling delayed start and sequential sampling When switching off a warning will sound and the display will show switching off while the MicroBio MB2 saves usa...

Page 17: ...lect the volume to be sampled by choosing a preset or manually adjust volume Press button to select preset option or to select manual adjustment Press START to confirm selection If Preset Volume is selected use or to select one of nine preset volumes then press START to begin sampling Take Sample Sample Method Preset Volume Sample Method Adjust Volume Select Preset 1 100L 16 ...

Page 18: ...e end of sampling the screen will display the total volume sampled and sound a warning tone and flash the top mounted LED To clear the warning press any key If left the unit will automatically switch off after 20 seconds To cancel sampling at any time press and hold the MENU button The unit will sound a short warning tone and display the volume sampled up to that point Air Volume 100L Sampled 10L ...

Page 19: ...is set as described in Take Sample Pressing START will start the delay timer and the screen will show the countdown During countdown the screen backlight will switch off to conserve battery power When the countdown reaches zero sampling will start The screen will display the volume sampled and remaining time When finished press any button or leave the MicroBio MB2 to automatically switch off Delay...

Page 20: ...quential Sampling How it works A cubic meter of air would normally be sampled in 10 minutes using a standard MicroBio MB2 Sequential sampling allows the user to configure the total volume to be sampled by using an adjustable volume or one of the user preset volumes over 5 minutes to 24 hours with optional delayed start if required To illustrate how sequential sampling works consider a 1 000 litre ...

Page 21: ...the sampling sequence the MicroBio MB2 displays a finished message and will automatically power off When the MicroBio MB2 is next switched on it will display a finished message with the total volume of air sampled Sequential Sampling Configuration Navigate to this option using MENU and press START Select the volume to be sampled by choosing a preset or manually adjust volume Press button to select...

Page 22: ...o be sampled then press START The volume may be set in the range of 10 to 10000 litres Next define the time over which sampling is to take place Use or to set the required time from 5 minutes to 24 hours then press START Enter the number of sequences 2 to 100 using or then press START Select Preset 1 100L Air Volume 100L Duration 08 00 00 Sequences 4 21 ...

Page 23: ...l volume has been sampled over the required time During sequential sampling the display will show a count of sequences top left the total volume taken bottom left time to next sample top right and time remaining until sequential sampling is complete bottom right At the end of sequential sampling the screen will indicate sampling is complete and the total sampled volume The MicroBio MB2 will automa...

Page 24: ...er is presented with Preset Volume 1 and the current set volume This value can be adjusted using or Users can set the volume between 10 and 10000 litres When the desired volume is reached press START to store and move on to the next preset When all required presets have been set press MENU to save and exit this option Settings are stored in non volatile memory and are retained when the battery is ...

Page 25: ...will exit this mode Battery Level Displays a percentage of battery capacity and voltage at rest The capacity is only an approximation and cannot be used to determine the exact remaining sampling capacity Power Diagnostics This information is only of use during service and repair of the sampler Unit Status Battery 100 V 6 21 Pack Vol 10000 Vh 0 00 Vl 0 00 24 ...

Page 26: ...amples have been taken by the MicroBio MB2 since the last factory reset This figure is useful for the manufacturer s maintenance and reliability statistics and may also be used as an indicator for the user s regular calibration regime Unit Version Displays the hardware and software revision information for the MicroBio MB2 It is used by the manufacturer for repair and maintenance purposes The soft...

Page 27: ...nts to zero Settings Unit settings include Language Battery Type and Auto power off time Press START to select this mode then or to navigate options Pressing MENU will exit this mode Pressing MENU at any time when in the settings options will also exit Settings Language When the language setting option is shown on the screen press START to select it Use or to select the required language then pres...

Page 28: ...o as to properly report on battery level The default setting is for NiMH cells as supplied with the MicroBio MB2 When the option is shown on the screen press START to select Use or to select the battery type then press START to store the setting Selecting the wrong type will not cause any harm to the MicroBio MB2 or the cells but will give incorrect reporting of capacity Settings Battery Type Batt...

Page 29: ...uired from 10 seconds to 5 minutes or disabled When the Auto off option is shown on the screen press START to select Use or to change the auto off time then press START to store To disable the automatic power off feature keep the button held until the display shows disabled Settings Auto off Auto off Time 60 s Auto off Disabled 28 ...

Page 30: ...n the contact plate or petri dish should be removed and sealed with its protective lid A note should be made on the lid regarding time location volume sampled etc and stored appropriately The plate should be incubated for a period of time and temperature dependant on the requirements of the media Once incubated the colony growths are then counted either manually or using an automatic colony counte...

Page 31: ...sidered as overloaded with micro organisms and the user should consider lower sampling volumes The colony concentration is the corrected count per volume of air sampled The results are normally expressed in colony forming units per cubic metre To convert the corrected count to CFU m3 use the equation Where nc is the corrected number of colonies counted and Vs is the sampled volume in litres For si...

Page 32: ...A 00068 The springs for both types have slots to allow a degree of adjustment to suit dish plate manufacturer variations To change the spring type remove the three screws as highlighted using the 2mm hex key supplied with the kit Lift off the support plate and springs then refit using the same screws support plate and the required springs Care must be taken not to over tighten or cross thread any ...

Page 33: ...ampling head it is recommended for optimum decontamination the MicroBio MB2 is set to sample at maximum volume allowing H2O2 vapour to be drawn through the head fan and exhaust Tests have been carried out by Bioquell UK Ltd on a range of sensitive electronic equipment to determine effects of such processes The conditions of these tests were Vapour sterilisation above the test conditions levels and...

Page 34: ...Scientific Limited part number A 00422 Calibration It is recommended all models of MicroBio MB2 are regularly calibrated in accordance with specific industry best practice Typically this will be on the anniversary of the instrument entering service The only calibration adjustment on the instrument is the flow rate Calibration is normally performed at an air pressure of 1013 mbar at 20ºC For calibr...

Page 35: ...ad area that keeps the head tight This may with time and use have moved Loosen the screw slide the spring to the edge of the sampling head plate and re tighten using the hex key supplied with the kit Do not over tighten the screw There is a nitrile O ring on the sampling head assembly that may have worn A replacement O ring is supplied with the kit part no A 00227 Q The contact plate or petri dish...

Page 36: ...4 54 62 94 122 134 206 15 15 55 63 95 124 135 208 16 16 56 65 96 126 136 211 17 18 57 66 97 127 137 213 18 19 58 67 98 129 138 216 19 20 59 69 99 131 139 219 20 21 60 70 100 133 140 222 21 22 61 71 101 135 141 224 22 23 62 73 102 136 142 227 23 24 63 74 103 138 143 230 24 26 64 76 104 140 144 233 25 27 65 77 105 142 145 236 26 28 66 78 106 144 146 239 27 29 67 80 107 146 147 242 28 30 68 81 108 14...

Page 37: ...4 58 94 107 134 163 15 15 55 59 95 109 135 164 16 16 56 61 96 110 136 166 17 18 57 62 97 111 137 167 18 19 58 63 98 113 138 169 19 20 59 64 99 114 139 170 20 21 60 65 100 115 140 172 21 22 61 66 101 117 141 174 22 23 62 68 102 118 142 175 23 24 63 69 103 119 143 177 24 25 64 70 104 121 144 178 25 26 65 71 105 122 145 180 26 27 66 72 106 123 146 181 27 28 67 74 107 125 147 183 28 29 68 75 108 126 1...

Page 38: ...acteria Human Flora Blood Agar 35 37 C Possible Pathogens Heart Infusion Agar Soya bean casein digest agar SCDA 35 37 C Environmental saprophytic SCDA or R2A 25 30 C Thermophylic EMB or Endo Agar 35 37 C Fungi Environmental saprophytes Malt Extract Agar MEA Room Temp Sabouraud Dextrose Room Temp Rose Bengal Agar RBA with streptomycin Inhibitory Mould Agar 20 25 C Xerophylic Malt Extract Agar with ...

Page 39: ...Hex key A 00206 Head screw pack A 00232 MB2 nitrile head O ring A 00227 Battery charger and cells A 00053 NiMH rechargeable cell pack A 00320 Mini tripod A 00061 Full height tripod A 00185 220 x 1 0mm stainless steel sampling head A 00021 400 x 0 7mm aluminium sampling head A 00020 400 x 1 0mm stainless steel sampling head HiFlow only A 00183 Hard carry case A 00060 38 ...

Page 40: ...al whilst airborne Bacterial cells when they become airborne normally rapidly die within a few seconds due to the evaporation of water associated with the particle Thus with higher humidity higher bioaerosol levels can prevail Airborne fungal cells yeasts moulds spores can remain viable for much longer periods even at low relative humidity and high or low temperature extremes These can pose health...

Page 41: ...00 litres per minute through a series of air inlets onto an agar filled 55 mm contact plate or 90 mm Petri dish up to a volume of 2 000 litres The agar media used should be chosen to suit the organisms which are being monitored For a wide range of bacteria use tryptic soy agar TSA casein soy peptone agar CPSA and nutrient agar NA There are other selective agars for more specific micro organisms Fo...

Page 42: ...on The first task is to run the connecting cable from where the sampling head is to be positioned to where the control unit is to be located A connector is pre fitted to the cable end to be connected to the remote sampling head 41 ...

Page 43: ...r lead free 1 x 2 5mm slot screwdriver The connector is assembled in the order as shown in the photographs below 1 Dismantle the connector by first removing the screw on the side of the connector 2 Slide the metal connector housing onto the cable end followed by the strain relief The strain relief will need pushing on firmly 42 ...

Page 44: ...has pin numbers embossed onto the surface adjacent to the pins i Pin 1 Red ii Pin 2 Blue 5 Fit frame onto cable end and refit cable clamp The clamp must clamp on the outer sheath heat shrink on the end of the cable Then solder the screen agains the soldier tab of the frame 43 ...

Page 45: ... the rim of the pin housing that sit into the holes on the frame Then replace the frame cover 7 Slide the assembly strain relief and metal shell together 8 Align shell so that the brass thread of the frame body can be seen then replace the housing screw and tighten 44 ...

Page 46: ...t only The MB2 RSH can be calibrated in the same manner as any other MicroBio bioaerosol sampler Disconnect the head and control unit from the cable and return to the manufacturer or approved agent for calibration Head units must be thoroughly cleaned sterilised and free from any potential contaminant before return to a calibration agent Documented evidence of cleaning must be provided Please info...

Page 47: ...NOTES 46 ...

Page 48: ...Cantium Scientific Limited MicroBio MB2 Bioaerosol Sampler Operating Manual www cantiumscientific com October 2019 Rev 5 A 00435 ...

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