Application hints
Instruction Manual, Feb-17
14
A general rule of thumb for HPLC-MS: Dilute the solutions you typically use for UV-
detection (approx. 1 mg/mL in e.g. methanol) by a factor of 1000 or 100, resulting in
concentrations of 1 to 10 µg/mL. Use flow injection analysis (FIA) of 0.1 µL and watch the
MS signal. If the MS signal is too small, inject 1 µL or more, until you get a clean mass
spectrum.
Fig. 6: Flow injection analysis FIA of 1 µL of 0.113 mg/mL diclofenac in methanol, upper
curve total ion current (TIC), lower curve single ion monitoring (SIM) of the masses
296.00, 297.00 and 298.00 dalton.
Fig. 7: Flow injection analysis FIA of 1 µL of 0.113 mg/mL diclofenac in methanol;
spectrum of diclofenac.
Use FIA to optimize MS parameters: Nebulizer pressure, drying gas temperature and
drying gas flow, capillary voltage and fragmentor voltage (consult the manufacturer’s
recommendations for your individual brand of HPLC-MS).
Optimizing TLC conditions
Summary of Contents for TLC-MS INTERFACE 2
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