43
BÜCHI
Fat Determination
B-820/B-821
5.2.7 Sample weight and mode adjustment
Position the cursor on <Sample weight/Mode>.
Manual sample weight
If no balance is connected to the B-820, the sample weights
must be entered manually in the sample weight table using
the keypad.
Confirm “Manual sample weight” with “ENTER”. The sample
weight table appears.
Automatic sample weight
For this option, a balance must be connected as described in
Chapter 4.4. Confirm “Automatic sample weight” with
“ENTER”. The sample weight table appears. After selecting
the mode, move the cursor to the next field (Weight [g] with
“ENTER”. When the weight has been transferred, the cursor
moves to the next field in a similar way to the “Manual sample
weight”.
Factors
The current factors are entered here. These valves are
incaporated in the final result. Confirm with “ENTER”. The
factor table will then appear.
Manual sample weight
When the sample weight table appears, the cursor will be
located at the first free sample number (No.). After selecting
the mode, enter the weight (in grams) for each sample and
the internal standards. Confirm each entry with “ENTER”.
Depending on the mode selected, the cursor will automatically
move to the next available field.
Automatic sample weight
When the sample weight table appears, the cursor will be
located at the first free sample number (No.). After selecting
the mode, transfer the weight (in grams) for each sample and
the internal standards for the connected balance type.
Depending on the mode selected, the cursor will automatically
move to the next available field.
Preheating
p H2
55kPa
Sample weight/Mode
p air
50kPa
Determination
T oven
130°C
Data manager
T inj.
220°C
Setup
T FID
260°C
Manual sample weight
Automatic sample weight
Factors
Main menu
5 Operation
No.
Mode
Weight Weight
Weight
Conc.
[g]
C13 [g]
C5 [g]
[%]
1
fact.gen.
0.7055 0.2115
99.8
Name:
Factor
2
Fact.b.a.
0.055
0.054
99.5
Name:
Factor C4
3
But.acid
0.8550 0.2134
0.056
Name:
Butter
4
gen.corr.
4.3575 0.1245
Name:
Wheat
5
spec.corr.
2.3654 0.2056
Name:
Cheese
Sample weight table
No.
Number of the sample in the sample weight table.
Mode
Determination mode used, which is selected dependent on the sample type ((See Chapter 3, Table 6). Select the mode with “OPTION” and
confirm with “ENTER”.
Weight [g]
Weight of the sample, which is entered in g. Confirm the weight with “ENTER”.
Weight C13 [g]
Weight of the internal standard (tridecanoic acid), which is entered in g. Confirm the weight with “ENTER”.
Weight C5 [g]
Weight of the internal standard (valeric acid), which is entered in g. Confirm the weight with “ENTER”.
Conc. [%]
Concentration of the fat standard (e.g., pork fat, 99.8% or concentration, depending on the standard used) and/or the butyric acid (e.g.,
butyric acid, 99.5% or according to purity used) that will be necessary for the determination of the factor. This column is only selected in the
modes “Gen.fact.”, “FA-fact.” and “Butyr.fact”. It is skipped over in all other modes.
Name
Sample identification (sample name) entered from the keypad and confirmed with “ENTER”.
Key for Sample weight table:
