12
BÜCHI
Fat Determination
B-820/B-821
3 Function
3.1
Operational principles
Figure 6: Diagram
햲
햳
햴
햵
햷
햶
헀
햿
햸
햾
햹
햽
햺
햻
헁
헂
햲
Synthetic air
햳
Hydrogen (percentage purity 5.0)
햴
Pressure reducing valve for synthetic air
햵
Pressure reducing valve for hydrogen
햶
Electronic valve for synthetic air
햷
Electronic valve for hydrogen
햸
Damping tube for synthetic air
햹
Damping tube for hydrogen
햺
Absorption tube
햻
Gas saturation vessel (formic acid 85 %)
햽
Forced-air oven
햾
Separation column
햿
Injection head
헀
Flame ionization detector (FID)
헁
Electronic
헂
Output
Main principle
1. Switch on the unit (the switch is located on the back of
the unit). Perform the input steps as described in Section
5 «Operation».
2. Using a syringe, extract some solution from the upper phase
of the extracted and saponified sample (see operating in-
structions for Extraction Unit B-815) and inject into the Fat
Determination Unit.
3. A temperature program is performed automatically accor-
ding to the selected method. The fatty acids are separated
from the solvent and internal standard and detected by
the flame ionization detector.
4. An evaluation program, specifically developed for this unit,
calculates the content of total fat of individual fatty acids.
