8
All parts (Figs. 1-5):
B
Screen module
C
LCD monitor
D
Tube
E
Lens turret
F
Objective
G
Slide (here: prepared slides)
H
Microscope table
I
Collector lens
J
LED lighting (transmitted light)
1)
Microscope foot
1!
Operating light
1@
On/ off switch for screen module
1#
LED top illumination (only for use with 4x objective)
1$
Stage
1%
Colour filter plate
1^
Focusing wheel
1&
Power connection
1*
Dimmer
1(
Lighting selector switch
2)
Input keys
2!
Photo button
2@
Menu key
2#
Change key
2$
Vernier scales
2%
Clamping screw
2^
Stage drive forwards/back
2&
Stage drive left/right
2*
Levers for clamping mounting plate
2(
Mounting plate clamp
3)
Clear aperture (without colour filter)
3!
Colour filter
3@
USB cable
3#
Box with 10 slides, 10 covers and 5 prepared slides
3$
A)Microscope utensils; B) Pipette; C) Tweezers
3%
Shrimp breeding enclosure
3^
Mikrotom tissue chopper
3&
Preparations: A) Yeast; B) Gum Arabic Media ; C) Sea salt;
D) Shrimp eggs
3*
Power supply plug
3(
Nylon case
1. Location, connection types
Before you begin with the assembly of your microscope, you must select a
suitable location.
First you should ensure that your microscope is set up on a stable, vibration-
free surface.
Power connection (220-230 V) is required for observations using the electrical
LED lighting and the electronic LCD eyepiece (screen module).
Furthermore the microscope possesses an interface to a PC (USB connection).
2. Start-up, electrical LED lighting
Before start-up, please check that the lighting selector switch (Fig. 1.19) is in
the “OFF position “.
The microscope is equipped with two LED lighting units. The lighting can take
place in three ways. At the selector switch (Fig. 1.19) select position “I”, in
order to illuminate the object from underneath (with transmitted light), or “II “,to
illuminate it from above (with top illumination). With position “III” the object can
be illuminated from above and below at the same time. The transmitted light unit
(Fig. 1.9) is used for transparent preparations (preparations on glass slides). In
order to see solid, non-transparent objects, select the top illumination unit
(Fig. 1.13). The top illumination unit is only useful when using the 4x-
objectivee. The use of both lighting at the same time is only useful with semi-
transparent objects. This mode of operation is not recommended for
transmitted light objects on slides, as this can lead to reflections on the slides.
To start-up, the power supply plug, which is provided, (Fig. 5.38) is connected
to the power connection (Fig. 1.17) at the rear of the microscope foot (Fig. 1.10)
and to a convenient outlet (220-230 V). Finally, switch on the desired lighting
using the lighting selector switch and set the required brightness with the
dimmer (Fig. 1.18).
Since your equipment is fitted with infinitely variable lighting (dimmer), the
optimum illumination of the object to be observed is ensured.
3. Observation
3.1 Microscope observation in general
Once you have assembled the microscope and appropriately adjusted the
illumination, the following principles apply:
a) Begin each observation with the lowest magnification, thus centring and
positioning of the object to be observed will be easier.
Move the microscope table(Fig. 1.7),using the focusing wheel (Fig. 1.16)
completely down and then turn the lens turret (Fig. 1.4) until it engages on the
lowest magnification (the 4x-objective is vertical).
b) Begin with a simple observation. Place a prepared slide (Fig. 1+3.6)
directly under the objective on the microscope table (Fig. 1+3.7), by clamping
it into the stage (Fig. 1+3.14). For this, move the lever for the clamping
mounting plate (Fig. 3.28) to the side, put the prepared slide onto the stage and
carefully allow the clamping mounting plate (Fig. 3.29) to return onto the
prepared slide. The object which is to be observed must be exactly above or
below the illumination. In order to achieve this, there are two milled thumb screws
on the stage. This is the so-called stage drive (Fig. 3.26+27). By turning
these screws it is possible to precisely position the object, in the left or right
direction (Fig. 3.27) and forwards or backwards (Fig. 3.26).
c) Then switch on the LCD monitor, by pressing the power switch on the screen
module (Fig. 1+2.12); the red operating light Fig. 1+2.11) will light up. Then look
at the LCD monitor (Fig. 1+2.2) and carefully turn the focusing wheel (Fig. 1.16)
until the image is sharp. With the stage you can now centre the object at the
desired place on the LCD monitor.
d) For higher magnification turn the lens turret (Fig. 1.4) to a higher setting
(objectives 10x and 40x).
Note:
Before you change the objective setting, first move the microscope
table fully downwards Fig. 1.7). This will avoid possible damage!
The higher the magnification, the more light is needed for a good image
quality.
Important notes:
Depending on the preparation in use, higher magnification in individual cases
will not lead to a better picture! The magnification is altered by changing the
objective. This alters the sharpness of the image which must be adjusted by
the focusing wheel (Fig. 1.16). Proceed here very carefully. If you raise the
microscope table too quickly, the objective and the slides can touch each
other and be damaged!
e) The magnification factor of the LCD eyepiece amounts to 10x. In
combination with the 4x-objective we obtain a total magnification of 40x, i.e. 1
mm of the object is shown as 40 mm on the LCD monitor. With the 10x
objective we have a total magnification of 100x, correspondingly, with the 40x
objective 400x.
