BJS Biotechnologies XXPRESS NGx, User Manual, Page: 35 / 57

P a g e | 35
XXPRESS® NGX USER MANUAL
– XP-5001-01-5
•
Once the xxplate™SBS is correctly inserted, push the door closed.
•
Touch the forward arrow on the bottom right of the screen to start
the experiment.
Figure 9
•
Once the experiment is initiated the xxpress
®
NGx performs several
checks on the plate. There will then be a short whirring sound as the
clamps lower onto the plate before it starts the heat cycling.
•
As the cycles complete the fluorescence measurements will appear
on the graph. The graph data can be adjusted using the filters on
the right. Touch the tiles to toggle the filters on and off.
▪
BASELINE SUBTRACT
uses the fluorescence readings of the
first 5 cycles to set the initial trace level to zero. This makes it
easier to compare multiple traces.
▪
SMOOTH
creates a best fit curve to the data.
▪
COLOUR COMPENSATION
is used for multiplex experiments. It
reduces the effects of cross-talk (interference) from adjacent
channels. It is not required for a single channel experiment.
•
Once all the thermal cycles have finished the xxpress
®
NGx will
display a message confirming that the
‘experiment is complete’, see
Figure 9a. Touch the tick to accept this. The results are saved