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XXPRESS® NGX USER MANUAL
– XP-5001-01-5
Figure 5a
•
Select the parameter to be changed. In figure 5a we have selected
the dwell time on step 1 of the PCR cycle. On selecting, the
parameter becomes highlighted. Type in the desired value using the
keyboard on the right of the display.
Once the user is happy with their changes they can save them by
touching the tick
symbol. Or if the user wishes to cancel and
lose their changes, touch the
cross.
•
In the example in Figure 5a there are 3 steps to the PCR. In step 1
the temperature is raised to 95°C using a ramp rate of 10°C per
second. This temperature is then held for 1 second.
•
In the second step, the temperature is reduced to 60°C, using a
ramp rate of 8°C per second. Once the temperature is at 60°C, this
is held for 5 seconds.
•
In the third step, the temperature is increased to 72°C, using a ramp
rate of 10°C per second. Once the temperature is at 72°C this is
held for 5 seconds. At the end of this dwell there is a fluorescence
measurement made using the channel 1 filter settings.
•
The temperatures for each step can be changed to any value
between 40°C and 99°C in 0.1°C steps.
•
The thermal ramp rate can be specified for each step independently
or set as a
GLOBAL RAMP
rate using the tile on the right of the pop-
Summary of Contents for XXPRESS NGx
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