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XXPRESS® NGX USER MANUAL
– XP-5001-01-5
•
The next screen controls the thermal profile required for the
experiment, see Figure 5. From the information already input into
xxpress
®
NGx,
the system automatically completes this screen with
the correct experiment structure and suggested starting values.
Figure 5
•
Looking at our example 3-Step PCR, we chose a SYBR Green
based test and Kapa SYBR 2G Fast Hot Start chemistry. In Figure
5 the thermal profile reflects this with an initial hot start at 95°C for
20 seconds followed by a 3-Step cycle which is repeated 40 times.
Finally, there is a cooling step to bring the plate down to ambient
temperature enabling safe removal from the unit.
•
Touching each part of the profile will produce a pop-up window
allowing the parameters to be modified, see Figure 5a. Here we
touched the Cycle 1 section.
Summary of Contents for XXPRESS NGx
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