Determining Optimal Concentration Conditions
25
P/N 128097 Rev. 00
TurboVap 96 User’s Manual
Biotage AB
Determining Optimal
Concentration Conditions
You should determine the settings that will best concentrate your
samples before operation. You should determine the desired:
•
Gas time (evaporation time)
•
Plate temperature
•
Flow rate
Considerations when Selecting Plate
Temperature
The two heaters operate over a temperature range of 20
C
(ambient) to 80
C ±5
C. The mild temperature range eliminates
“hot” spots and improves sample recovery for more volatile
compounds.
When selecting the
Plate Temperature
, consider the following:
•
Faster evaporation occurs as the plate temperature is
increased; however, highly volatile analytes can be lost if
allowed to sit for extended periods of time in a hot environment.
•
You can use a Plate Temperature above the boiling point of your
solvent if the gas flow rate is high enough to create a cooling
effect over the solvent.
•
The cooling effect of both the gas flow and the resulting
evaporation of a solvent will keep the internal temperature
below that of the set plate temperature.
•
The temperature of each well during the evaporation process
will be below the set plate temperature. Once the liquid in the
well evaporates and the gas flow stops, the well temperature will
rise.
When the workstation is turned on, the plate temperature warms up
to wait for the next evaporation process. If the cover is opened after
the evaporation process begins, the following chart shows how the
temperature changes.
State of
workstation
Cover
Plate Temp
setting is < 37
C,
system runs at …
Plate Temp
setting is > 37
C,
system runs at …
Turned on and
initialized.
Either open or
closed
Set temperature
37
C
Evaporation has
started
Closed
Set temperature
Set temperature
