Biotage Selekt, User Manual, Page: 9 / 28

7
Set Up a Purification
Figure 14. The Column panel expanded.
Specify the Collection Parameters
»
λ -All * : Select whether the average absorbance within
a user-defined wavelength range should be used for
collection and fractionation (
Collect ), used for monitoring
( Show ), or not be shown during the run ( Hide ).
»
UV1 and UV2:
Select whether the wavelength signals should
be used for collection and fractionation ( Collect ), used for
monitoring ( Show ), or not be shown during the run ( Hide ).
»
Wavelengths * :
The shortest and longest wavelength to
be included in the
λ
-All signal. The range is 200 to 400
nm (UV detector) or 198 to 810 nm (UV-VIS detector).
»
Wavelength:
The wavelength to be used for
UV1 and UV2. The range is 200 to 400 nm (UV
detector) or 198 to 810 nm (UV-VIS detector).
»
Threshold:
Used to collect samples with an absorbance
exceeding the set absorbance threshold.
»
Collect All: Used to collect the entire run.
»
UV Baseline Correction
*
: When this option is turned on, the
gradient run is preceded by a light absorbance detection
phase. During this phase, the light absorbance of the used
chromatography solvents is measured for all wavelengths
that will be used for collection and fractionation (UV1,
UV2, and/or
λ
-All). The measurement results in a baseline
containing the maximum absorbance of the solvent. During
the gradient run, the baseline is subtracted from the signal.
*
Only available on systems with a Spektra software license.
Specify the Rack Parameters
The Selekt collection racks have RFID tags that are
automatically identified by the system when placed on the
collection bed.
If the RFID rack detection feature is disabled in the system
settings (see page 15), it is possible to select the collection
rack manually by expanding the
Rack panel (see Figure 15).
If you want to use a lower fraction volume than the one defined
for the selected rack(s), expand the
Rack panel, enable the
Max Fraction Volume option, and enter the desired volume.
Note that this setting will only be applied on racks with a higher
vessel volume.
Figure 15. The Rack panel expanded on a system with extended
collection bed.