Biotage Selekt, User Manual, Page: 5 / 28

3
Prepare the System
Inspect the Tubing
Warning
»
Shut down the system before replacing any tubing. Use only
tubing designed for the Selekt system and supplied by Biotage.
Inspect all tubes before each run to ensure that they do not
show signs of wear or damage and that they are properly
connected and tightened. Use caution when finger tightening
fittings to prevent stripped threads or crushed ferrules.
All external tubing on the system except for the tubing on the
collection arm can be replaced by the user.
Note:
All tube types, dimensions, and lengths are essential
for the performance of the system. Only replace tubes with the
equivalent tubes designed for the Selekt system and supplied
by Biotage.
Mount Column Holders
The system is shipped with column holders of different sizes.
Ensure that they are mounted in the desired positions on the
system. Figure 5 shows how to mount a column holder on
the right side of the system. There are also two positions for
column holders on the front of the system that can be used for
Biotage columns up to 50g; see Figure 6.
Figure 5. Slide the column holder screw into the desired position on
the right side of the system and then place the holder over the screw
and fasten.
Figure 6. Biotage columns up to 50g can be placed on the front of
the system.
Prepare the System
Start Up the System
Turn on the system using the power switch located on the left
side of the system; see Figure 7.
Figure 7. The power switch is located on the left side of the system.
Assign Solvents
When a purification is run, the software determines which
solvent inlets are connected to the chromatography solvents
used in the run.
Note: For reversed-phase purification with methanol and
water, it is strongly recommended to premix the water with
5% of methanol and degass either through vacuum or
sonication. Degassing of protic solvent blends decreases
out-gassing of entrapped air during gradient elution, which
will impact gradient performance and flow rates.
Note: All four solvent inlets must be primed with solvent to
achieve the specified pump performance.
1. In the software, select Solvent Setup from one of the
solvent drop-down lists in the gradient view. The Solvent
Setup
window opens; see Figure 8.
2. Assign a solvent to each solvent inlet using the solvent
drop-down lists.
To add solvents to the list, select
Solvent Administration .
For more information, see “Manage Solvents” on page 13.
3. Prime the solvent inlets that have been assigned a new
solvent; see page 4.