Biotage
®
Selekt
User Manual
Page 1: ...Biotage Selekt User Manual ...
Page 2: ...fication 10 Unload a Purification 10 Flush the Column with Air 10 Purge the Column 10 Unload the Run 10 Flush the System 10 Shut Down the System 11 Results 11 Export a Report to USB 11 Base a New Run on a Previous Run 12 Flushes and Purge 12 Flush 12 Air Flush 12 Purge 13 Data Administration 13 Manage Column Types 13 Manage Solvents 14 Manage User Accounts 15 System Settings 15 System 15 Purificat...
Page 3: ...mL 35 positions for 16 x 100 mm tubes with a vessel volume of 14 mL 35 positions for 16 x 150 mm tubes with a vessel volume of 22 mL 28 positions for 18 x 150 mm tubes with a vessel volume of 27 mL 15 positions for 25 x 150 mm tubes with a vessel volume of 45 mL 6 positions for 120 mL flasks 10 positions for 240 mL flasks 8 positions for 480 mL flasks The collection bed can either hold a collectio...
Page 4: ...ation Possible collection and fractionation parameters are Collect All Threshold and Valley Fractionation Only available on systems with a Spektra software license UV1 and UV2 The system uses one or two wavelength signals for collection and fractionation Possible collection and fractionation parameters are Collect All Threshold and Valley Fractionation All collection methods fractionate on volume ...
Page 5: ...right side of the system and then place the holder over the screw and fasten Figure 6 Biotage columns up to 50g can be placed on the front of the system Prepare the System Start Up the System Turn on the system using the power switch located on the left side of the system see Figure 7 Figure 7 The power switch is located on the left side of the system Assign Solvents When a purification is run the...
Page 6: ...he previous purification and fill them with new solvents Note All four solvent inlets must be primed with solvent to achieve the specified pump performance Note We recommend to have all solvent reservoirs on the same physical height to improve accuracy in the solvent mixing 1 In the software select Solvent Setup from one of the solvent drop down lists in the gradient view The Solvent Setup window ...
Page 7: ...ystem when placed on the collection bed If automatic rack detection has been disabled see page 15 select the collection rack manually see Specify the Rack Parameters on page 7 7 Set up the run parameters as described in the following sections Note An invalid setting is highlighted with a red frame see Figure 10 press in the bottom right corner for more information Figure 10 The run setup view Spec...
Page 8: ...liding the Front line to the correct position 4 Enter the Rf value of the product of interest in the Rf Product text box or by sliding the Product line to the correct position on the TLC plate 5 Enter the Rf value of the impurity closest below and above the product in the Rf Impurity 1 and Rf Impurity 2 text boxes or by sliding the corresponding lines to the correct positions on the TLC plate A va...
Page 9: ...d on the gradient run is preceded by a light absorbance detection phase During this phase the light absorbance of the used chromatography solvents is measured for all wavelengths that will be used for collection and fractionation UV1 UV2 and or λ All The measurement results in a baseline containing the maximum absorbance of the solvent During the gradient run the baseline is subtracted from the si...
Page 10: ...for the whole detector range are displayed in the run view see Figure 16 Expand a graph or the gradient table by pressing Change the length unit on the chromatogram by selecting CV mm ss or mL from the drop down menu in the gradient table Fractions Fractions that are already collected can be located by matching their colors and numbers on the chromatogram with the vessel colors and numbers in the ...
Page 11: ...etector flush if enabled To enable or disable flushes and specify whether enabled flushes are collected or not see page 15 Auto Extend If the collection criteria are still met light absorption is above threshold level when the system nears the end of a purification the system extends the gradient purification stage of the run with 25 of the total gradient length using the final conditions of the r...
Page 12: ...hat you assign methanol or a similar solvent to the inlet line used with the halogenated solvent see page 3 and flush with at least 30 mL see page 12 Unload a Purification Shut Down the System Warning Failure to perform an orderly system shutdown may result in user data corruption and or remaining pressure An orderly system shutdown helps prevent data corruption For critical applications use of a ...
Page 13: ...a chromatogram and TLC data if entered in the TLC to Gradient editor Figure 19 The report view Export a Report to USB To save a report as a PDF file on a USB memory device 1 Connect the memory device to one of the two USB ports located on the left side of the system see Figure 18 2 Select the desired report 3 Press Export The report is saved as a PDF file at USB biotage selekt user name Base a New...
Page 14: ...together 3 Enter the volume in the Volume text box 4 Enter the flow rate in the Flow Rate text box Note that maximum flow rate that can be used depends on the column type if used and the maximum aspiration rate s for the used solvent s 5 Enter the percentage to be used of each solvent connected to the system If several solvents are listed and you only want to use one solvent enter 100 for that sol...
Page 15: ...ibration Length The equilibration length in CV Equilibration Flow Rate The equilibration flow rate in mL min Air Flush Length The air flush length in CV This value will be used as the default volume for the air flush feature see page 12 Load Capacity at ΔCV 2 The approximate load capacity when delta CV is 2 Chemistry Type The kind of chromatography the column can be used for normal or reversed pha...
Page 16: ...nd in other user name selection boxes Name The user name will be used in the various user name selection boxes as well as in the purification reports If you change the user name all result records associated with the user will be updated Password It is possible to password protect a user account with System Owner privilege The password will be used when entering the Data Administration System Sett...
Page 17: ...d The system has to be restarted for the new configuration to take effect Purification Note Collected flushes are added to the gradient in the report Line Flush If enabled the system flushes the column inlet line at the end of the run using the run s weakest solvent Select whether to Collect the flush using the same collection criteria as during the gradient run or send it To Waste Note If the aut...
Page 18: ...ith water If required a small amount of mild soap may also be used After cleaning wipe dry with a clean lint free cloth Clean the Flow Cell of the Internal UV Detector Warning Ultraviolet UV light can injure your eyes Always turn off the system before removing the flow cell Never have the system turned on when the flow cell is removed or when the retaining nut is loosened Note Always handle the fl...
Page 19: ...riodic UV or UV VIS signals can be signs of stuck check valves Warning When releasing a stuck check valve there is a risk of small amount of solvent splashing out 1 Release the pressure by slowly unscrewing the check valve cap from one of the CV OUT valves using the Torx 50 screwdriver supplied with the system see Figure 28 2 Remove the check valve by pushing an unused pipette tip or similar into ...
Page 20: ... spare parts is available at www biotage com Note All tube types dimensions and lengths are essential for the performance of the system Only replace tubes with the equivalent tubes designed for the Selekt system and supplied by Biotage If an external leakage is observed 1 Shut down the system as described above 2 Disconnect the power cord from the power outlet 3 Remove the spillage using the appro...
Page 21: ...ut down the system as described on page 10 2 Remove the needle 3 Assemble the new needle ferrule and peek nut P N 411915 Ensure that the needle is flush with the end of the ferrule see A in Figure 33 4 Mount the needle on the collection arm Ensure that the needle is touching the needle guide see B in Figure 33 Figure 33 A B Assemble the needle ferrule A and nut and assemble it on the collection ar...
Page 22: ...d gradient A longer gradient delay may be due to interactions with the silica where strong solvent is being selectively retained on the column 2 The gradient as observed by the internal detector will at times decline and plateau before the programmed gradient does This is due to the detection limit of the internal detector Any increase in the concentration of strong solvent will not be registered ...
Page 23: ...yed in the top left corner of the software fasten the check valve cap Figure 34 Removing the check valve cap from one of the CV OUT valves Pump Related Problems Note If using highly volatile i e high vapor pressure solvents such as DCM reservoir elevation is strongly recommended Have all solvents on the same physical height to improve accuracy in the solvent mixing It is also highly advisable to r...
Page 24: ...ime the Solvent Inlets on page 4 Stuck pump check valve s Flush with methanol or a similar solvent see page 12 Blockage in solvent line s Sonicate or replace the solvent inlet filters see page 19 Straighten or replace kinked tubing If this does not solve the problem flush the entire system with methanol or isopropanol at a low flow rate see page 12 Leakage Check for leaks in the tubing or fittings...
Page 25: ...Dichloromethane DCM 75 09 2 0 42 5 V 235 6 9 475 3 Ethanol 64 17 5 0 88 2 II 210 1 3 90 0 Ethyl acetate 141 78 6 0 58 6 VIa 255 1 4 98 3 n Heptane 142 82 5 0 01 0 210 0 7 47 4 n Hexane 110 54 3 0 01 0 210 2 3 161 6 Methanol 67 56 1 0 95 2 II 210 1 9 129 7 2 Propanol IPA isopropanol 67 63 0 0 82 2 II 210 0 6 44 0 Tetrahydrofuran 109 99 9 0 57 3 III 220 2 5 172 4 Toluene 108 88 3 0 29 7 VII 286 0 4 ...
Page 26: ... below Part No Description Qty 415115SP Inlet Check Valve 4 415625SP Flow Cell 1 411915 Fraction Collector Needle 1 411916SP Fuse 4 0 TA 250 VAC 5 x 20 mm 5 413008SP Four solvent inlet lines and filters S1 S4 1 411851SP Waste outlet tube 1 Manufacturer Biotage Sweden AB Contact Us Biotage Sweden AB Box 8 SE 751 03 Uppsala SWEDEN Visiting address Vimpelgatan 5 Phone 46 18 56 59 00 Fax 46 18 59 19 2...
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Page 28: ...m Part Number 416183 A 2018 Biotage All rights reserved No material may be reproduced or published without the written permission of Biotage Information in this document is subject to change without notice and does not represent any commitment from Biotage E OE A list of all trademarks owned by Biotage AB is available at www biotage com legal Other product and company names mentioned herein may be...
