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Assign or modify reagents
Only Administrative users can assign or modify Reagents in the Reagents Manager.
Example Setup
To better illustrate how to setup and perform a run, an example scenario is provided. You may use this tool as a
quick-start procedure to familiarize yourself with the workflow process. See product datasheet for protocol details.
• Pre-Treatment:
Reveal
• Block:
Peroxidazed 1 (Cat. No. PX968) for 5 min
• Antibody:
Ki-67 (M) (Cat. No. IP375)
• Detection:
MACH 3 Mouse AP Polymer Detection (Cat. No. M3M532)
• Secondary:
MACH 3 Mouse Probe (MP530) for 10 min
• Tertiary:
MACH 3 Mouse AP-Polymer (MAP532) for 10 min
• Chromogen:
iP Warp Red (Cat. No. IPK5024) for 7 min
• CounterStain:
iP FLX Hematoxylin (Cat. No. IPCS5006) for 5 min
1.
From the
Module Selection
menu select
Reagent Manager
Module. Press
Execute
to proceed.
2.
Go through each
Reagent Group
and set up the appropriate reagents and conditions.
2a. Pre-Treatment Group
• Select
Pre-Treatment
from Reagent Group at the left side of the screen.
• Select
Reveal
as your pre-treatment reagent.
Note: If you would like to add a new pre-treatment reagent not in the list, first select a
Sub Group
(Antigen
Retrieval or Enzyme), then select the
Add
button.
