3-4
Bio-Tek FL600
Figure 3-4: Removing the cover on the emission filter wheel
Step 5:
Remove the filters you wish to change, being careful not to touch
the filter’s glass surface, or other optical components. Use extreme
care to avoid dropping the filter into the interior of the instrument.
Rotate the filter wheel until you reach the filter you want to
change.
Using filter forceps (available in the Accessory Kit),
remove the filter cell by pulling it straight up and out of the
wheel.
If removing absorbance filters, remove the holder
containing the filter, not the filter itself.
Step 6:
Ensure that the glass surfaces of the filters are free of dust and
smudges.
Step 7:
Insert the new filter, and note its wavelength and position on the
filter wheel.
Fluorescence Filters:
Align the new filter with the filter wheel, using the
illustration below (Figure 3-5). The arrow on the filter cell
should point away from the center of the filter wheel.
Insert the new fluorescence filter, and seat the filter by
applying gentle, equal pressure on the edges of the filter
housing.
Helpful hint: If the filter is marked 485/20X:
•
485 is the center wavelength;
•
20 is the bandwidth at one-half maximum transmission
(BWHM) of the excitation (X) filter.
