Berthold 55393 BA2, Operating Manual

Page: 41 / 126

LB 983 NightSHADE Operating Manual Quick Start
41
You can either select an already existing format using the load button or define a new sample
format. The following parameters are required:

Sample Size :
The sample size refers to the image size (width) in mm. To make sure that the sample will
be measured completely it is recommended to define the image size a little bit larger than
the actual size of the sample

Sample Height:
The sample heights refers to the focus height. If you are measuring flat samples (gels,
blots) located on the base plate the sample height is 0 mm. If elevated samples are meas-
ured (e.g. animals or plants) the sample height has to be set to a relevant range (e.g. height
of a special leaf). If you are analyzing animals, it is advisable to use half the height of the
animal as sample height. If you are working with sample holders or other extra parts (e.g.
transilluminators), you have to take their height into account when calculating the sample
height.

Comment:
You can enter a comment on the sample format which describes the
format.
The
can be used to start an acquisition using the entered values. The result is shown
in a preview where you can check the focus or whether the entered sample size is adequate for
the acquisition of the sample.
To go to the next page select
.
On this page you can set up the
“Region of Interest”
settings as well as the
“
Multi Sample
”
parameters.