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Beckman Coulter TQ-Prep, Reference

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PN 4237395AA 

 B-9

OPERATOR CERTIFICATION PROCEDURE

PIPET CALIBRATION PROCEDURE

B

10

Repeat steps 3 through 9 until 10 results 
are listed.

11

Calculate the mean and the standard 
deviation (SD).

 r

The mean must be 100 ±0.5. Adjust 
the pipet’s volume to dispense if the 
mean is not correct.

 r

The SD must be less than 1.0. This 
represents your skill level. Repeat 
this procedure until the SD is less 
than 1.0.

Part I - Pipet Precision (Weight of water dispensed)

Pipet Type:                         ____ Air Displacement       ____ Positive Displacement

1. __________mg

2. __________mg

3. __________mg

4. __________mg

5. __________mg

6. __________mg

7. __________mg

8. __________mg

9. __________mg

10. _________mg

Mean _______mg

SD _________mg

1. __________mg

2. __________mg

3. __________mg

4. __________mg

5. __________mg

6. __________mg

7. __________mg

8. __________mg

9. __________mg

10. _________mg

Mean _______mg

SD _________mg

1. __________mg

2. __________mg

3. __________mg

4. __________mg

5. __________mg

6. __________mg

7. __________mg

8. __________mg

9. __________mg

10. _________mg

Mean _______mg

SD _________mg

COULTER

®

 TQ-PREP

™

 Workstation Operator Certification Form

100.1

100.0

100.0

99.8

99.9

100.1

100.0

100.1

100.0

99.8

Part I - Pipet Precision (Weight of water dispensed)

Pipet Type:                         ____ Air Displacement       ____ Positive Displacement

1. __________mg

2. __________mg

3. __________mg

4. __________mg

5. __________mg

6. __________mg

7. __________mg

8. __________mg

9. __________mg

10. _________mg

Mean _______mg

SD _________mg

1. __________mg

2. __________mg

3. __________mg

4. __________mg

5. __________mg

6. __________mg

7. __________mg

8. __________mg

9. __________mg

10. _________mg

Mean _______mg

SD _________mg

1. __________mg

2. __________mg

3. __________mg

4. __________mg

5. __________mg

6. __________mg

7. __________mg

8. __________mg

9. __________mg

10. _________mg

Mean _______mg

SD _________mg

COULTER

®

 TQ-PREP

™

 Workstation Operator Certification Form

100.1

100.0

100.0

99.8

99.9

100.1

100.0

100.1

100.0

99.8

99.98

.11352924

Summary of Contents for TQ-Prep

Page 1: ...COULTER TQ Prep Workstation Reference PN 4237395AA June 2010 Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 ...

Page 2: ...ooting To avoid injury r Keep doors covers and panels closed and secured in place while the instrument is in use r Take full advantage of the safety features of the instrument Do not defeat safety interlocks and sensors r Acknowledge and act upon instrument alarms and error messages r Keep away from moving parts r Report any broken parts to your Beckman Coulter Representative r Open remove and clo...

Page 3: ...revision are indicated in text by a bar in the margin of the amended page This document applies to the latest software listed and higher versions When a subsequent software version changes the information in this document a new issue will be released to the Beckman Coulter website For labeling updates go to www beckmancoulter com and download the most recent manual or system help for your instrume...

Page 4: ...PN 4237395AA iv REVISION STATUS ...

Page 5: ...BOUT THE REFERENCE MANUAL x CONVENTIONS x 1 USE AND FUNCTION 1 1 1 1 INTENDED USE 1 1 1 2 METHOD HISTORY 1 1 1 3 SYSTEM COMPONENTS 1 2 Sample Processing Area 1 2 Carousel 1 2 Indexing Base 1 2 Dispensing Head 1 2 Tube Detector 1 3 Tube Lifter Vortex Mixer 1 3 Reagent Delivery System 1 3 Electronics 1 3 Reagent Storage and Dispense Area 1 3 Touch Screen 1 4 1 4 OPTIONS 1 4 LALS OPTION 1 4 1 5 REAGE...

Page 6: ...LLATION 2 2 Inspecting After Delivery 2 2 Unpacking and Checking Instrument 2 3 Connecting the OPTION Reagent Container 2 6 Connecting the Reagent A B and C Containers 2 9 Connecting the WASTE Container 2 14 Powering On the Instrument 2 17 Checking Carousel Alignment 2 19 Priming and Preparing the Instrument for Use 2 21 3 OPERATION PRINCIPLES 3 1 3 1 GENERAL PRINCIPLES 3 1 3 2 NORMAL SAMPLE FLOW ...

Page 7: ...Processing Time 4 2 4 2 PERFORMANCE SPECIFICATIONS 4 3 Accuracy of Delivery Volume of Distilled Water 4 3 Precision Reproducibility 4 3 Calibration 4 3 4 3 PERFORMANCE CHARACTERISTICS 4 3 A LOG SHEETS A 1 B OPERATOR CERTIFICATION PROCEDURE B 1 B 1 PURPOSE AND SCOPE B 1 B 2 OPERATOR CERTIFICATION PROCEDURE B 1 B 3 PIPET CALIBRATION PROCEDURE B 1 With Air Displacement Pipet Reverse Mode B 2 With Pos...

Page 8: ...PN 4237395AA viii CONTENTS ...

Page 9: ...PN 4237395AA 9 1 1 COULTER TQ Prep Workstation 1 1 1 2 Sample Processing Area 1 2 1 3 Reagent Storage Area with OPTION Reagent 1 4 3 1 Carousel Home and Tube Loading Positions 3 1 ...

Page 10: ... instrument Read the Controls and Indicators chapter to become familiar with the different parts of your system Then go through the detailed step by step procedures of daily startup processing samples and shutdown The calibration chapter contains the gravimetric validation and precision tests The remaining chapters contain the cleaning replacement and adjustment procedures and troubleshooting tabl...

Page 11: ... normal sample flow on the instrument S Chapter 4 Specifications Characteristics Details the instrument and performance specifications and the performance characteristics S Appendices The appendices provide reference material on the following topics r Log Sheets r Operator Certification S References References used throughout the manuals S Glossary Definitions of words abbreviations and acronyms u...

Page 12: ...emove unbound antibody and debris The repeated trauma of mixing vortexing and centrifugation can selectively damage malignant or activated cells In addition nonrandom cell loss can occur by adherence to containers or failure to form a complete pellet during centrifugation 4 Most procedures are labor intensive require exacting technique and become unmanageable when processing large numbers of sampl...

Page 13: ...ccur with the lid closed Figure 1 2 Sample Processing Area Carousel Two sample carousels each with a handle and 32 test tube slots are provided with each unit The key located on the carousel aligns with the hub key on the indexing base to correctly position the carousel for sample processing Indexing Base The indexing base moves the carousel to its home position and moves the carousel during seque...

Page 14: ...icrocontroller located on the Control Interface card controls the motors sensors syringes the cover lid interlock switches reagent level sense probes and an internal communication link to the system microprocessor assembly A universal input power module supplies all the necessary dc voltages to the system components Reagent Storage and Dispense Area The reagent storage and dispense area is covered...

Page 15: ...alents The TQ Prep Workstation is designed for use with the ImmunoPrep Reagent System All stated performance characteristics in this manual are based on the use of the TQ Prep Workstation with these reagents Refer to the reagents package insert for detailed information before using the reagents Reagent A ImmunoPrep A a lysing reagent causes simultaneous rapid destruction of the erythrocytes while ...

Page 16: ...if your system has the LALS OPTION This reagent is for use on flow cytometers to allow the measurement of a low angle of forward scatter light 1 6 MATERIAL SAFETY DATA SHEETS MSDS To obtain an MSDS for Coulter reagents used on the TQ Prep Workstation 1 In the USA either call Coulter Customer Operations 800 526 7694 or write to Coulter Corporation Attn MSDS Requests P O Box 169015 Miami FL 33116 90...

Page 17: ...PN 4237395AA 1 6 USE AND FUNCTION MATERIAL SAFETY DATA SHEETS MSDS ...

Page 18: ...space on the left side to remove reagent cover and replace reagent containers If your instrument is located in a safety cabinet laminar flow hood you can remove the reagent cover permanently Electrical Input CAUTION Do not use an extension cord Overheating melting and burning of the extension cord can occur if you use an extension cord Plug the power cord directly into an electrical outlet Positio...

Page 19: ...insured separately file a claim with the insurance company Operator Certification After the system is installed each operator should perform the Operator Certification procedure in Appendix B before running samples 2 3 CUSTOMER INSTALLATION Inspecting After Delivery CAUTION Possible instrument damage could occur if you improperly uncrate the instrument install it or set it up incorrectly If you ha...

Page 20: ... 2 Unpacking and Checking Instrument 1 Open the carton and remove the instrument Note It is easier if two people lift the instrument out of the carton 2 Remove the foam packing material unwrap the plastic packing and set the instrument on a flat surface ...

Page 21: ...PN 4237395AA 2 4 INSTALLATION CUSTOMER INSTALLATION 3 Lift the sample processing area lid 4 Remove the foam packing material 5 Close the sample processing area lid ...

Page 22: ...ION CUSTOMER INSTALLATION 2 6 Check the fittings at the top of the syringes for visible damage The connections should be finger tight See the Replacing the Syringe procedure in Chapter 7 in the Operator s Guide for more details ...

Page 23: ...through 6 to connect the OPTION reagent container r If your instrument does not have the LALS OPTION proceed to Connecting Reagent A B and C Containers 2 r Remove the empty OPTION reagent container and cap assembly from its packaging r Check the cap assembly fittings for visible damage 3 Unscrew the cap assembly from the empty OPTION reagent container ...

Page 24: ... 4237395AA 2 7 INSTALLATION CUSTOMER INSTALLATION 2 4 Fill the OPTION reagent container with distilled or deionized water 5 Screw the cap assembly with the white fitting onto the OPTION reagent container ...

Page 25: ... 4237395AA 2 8 INSTALLATION CUSTOMER INSTALLATION 6 Place the OPTION reagent container in the instrument 7 Screw the tubing connector from the OPTION reagent onto the side fitting of the OPTION syringe P ...

Page 26: ...t room temperature Make sure the reagents are at room temperature before they are placed in the instrument 2 Remove the reagent cap from a reagent Save the cap for later 3 Remove the protective seal so that the cap assembly s level sensor probe and tubing fit easily through the container opening COULTER IMMUNOPREP F C 16 61 30 86 ImmunoPrep Leukocyte Preparation System ...

Page 27: ...sembly from its packaging 5 Unscrew the cap assembly from the empty reagent container Save the empty container for step 11 Note The cap assembly fittings are color coded r Green Reagent A r Blue Reagent B r Yellow Reagent C r Red WASTE r White OPTION Reagent 6 Screw the cap assembly onto the full reagent container ...

Page 28: ...5AA 2 11 INSTALLATION CUSTOMER INSTALLATION 2 7 Place the full reagent container in the instrument 8 Screw the tubing connector from the reagent container onto the side fitting of the corresponding syringe A B C ...

Page 29: ...match the cap assemblies r Red WASTE r Green Reagent A r Blue Reagent B r Yellow Reagent C 10 Connect the level sense connector from the cap assembly to the instrument by a Lining up the two red dots r Red dot on the level sense connector r Red dot on the corresponding color coded opening b Pushing the level sense connector into the opening A B C WASTE ...

Page 30: ...ATION 2 11 Screw the saved cap onto the empty reagent container Save this container for future use such as in the long term shutdown procedure 12 Repeat steps 2 through 11 for the other two ImmunoPrep reagents ImmunoPrep Leukocyte Preparation System ...

Page 31: ... INSTALLATION CUSTOMER INSTALLATION Connecting the WASTE Container 1 Remove the WASTE container and its cap assembly from its packaging Check that the cap assembly is on tight 2 Locate the tubing taped to the instrument ...

Page 32: ...PN 4237395AA 2 15 INSTALLATION CUSTOMER INSTALLATION 2 3 Insert the tubing into the opening on the WASTE container Screw in the red connector 4 Place the WASTE container in the instrument ...

Page 33: ...ing up the two red dots r Red dot on the level sense connector r Red dot on the corresponding color coded opening b Pushing the level sense connector into the opening 6 Remove the reagent cover from its packaging and place it on the instrument Note If your instrument is located in a safety cabinet laminar flow hood you do not need to use the reagent cover ...

Page 34: ...ER INSTALLATION 2 Powering On the Instrument 1 Plug the power cord into the electrical receptacle at the back of the instrument and then into the wall receptacle 2 Turn on the power switch 3 Check that the front power indicator light is on ...

Page 35: ... appears briefly on the touch screen 5 Check that r The Main screen appears r No troubleshooting icon appears on the Main screen If a troubleshooting icon does appear go to Chapter 8 in the Operator s Guide to correct the problem 6 Adjust the contrast of the touch screen for best viewing angle as needed ...

Page 36: ...ousel Alignment 1 Open the sample processing area lid 2 Place an empty carousel onto the indexing base Note To correctly secure the carousel line up the arrow on the carousel above the hub key on the indexing base before placement 3 Close the sample processing area lid ...

Page 37: ...ousel completes processing if any of these conditions occur then you need to align the carousel as described in Chapter 7 of the Operator s Guide t or appears on the touch screen t The instrument pauses and beeps r If none of the above conditions occur continue with installation ...

Page 38: ...f your system has the LALS OPTION select the correct reagent mode 3 or 4 reagents Note The displayed mode is the active mode 2 Press 3 Press to prime the instrument with reagents and to set the reagent counter to 300 r The Prime screen appears during priming r When priming is done the Main screen appears ...

Page 39: ...rator s Guide Note If your test tubes meet the specification criteria in Chapter 4 and none of these three troubleshooting icons or appears on the touch screen during this test then your tubes are compatible with the instrument 5 Each system operator should perform the Operator Certification procedure in Appendix B before running samples ...

Page 40: ...e integrity 4 r Cell loss due to washing and centrifugation is eliminated Because these steps are not required processing time is substantially reduced r Automation further provides reproducible standardized sample preparation 3 2 NORMAL SAMPLE FLOW 1 At power up startup diagnostics are run on the TQ Prep Workstation This includes r Moving all of the syringe plungers up and then down to fill the s...

Page 41: ...ronically controlled syringe adds 265 µL of ImmunoPrep Reagent B to the sample At the completion of the reagent dispensing the vortex mixer mixes the sample for 10 seconds 9 The vortex mixer stops mixing and an electronically controlled syringe adds 100 µL of ImmunoPrep Reagent C to the sample At the completion of the reagent dispensing the vortex mixer mixes the sample for 10 seconds 10 In system...

Page 42: ...ng from the syringes to the reagent containers r See Chapter 4 Specifications Characteristics for amounts of dispensed reagent r Press to request this prime Automatic This prime dispenses 20 µL of each reagent automatically when you request a function sample processing sample processing with preincubation Gravimetric Validation user requested prime or new reagent prime after the instrument has bee...

Page 43: ...PN 4237395AA 3 4 OPERATION PRINCIPLES TYPES OF PRIMING ...

Page 44: ...g Temperature 10 35 C 50 95 F Humidity 30 85 without condensation Recommended Reagents r The COULTER ImmunoPrep Reagent System which contains t ImmunoPrep A erythrocyte lysing reagent t ImmunoPrep B leukocyte stabilizing reagent t ImmunoPrep C cell membrane fixing reagent r Distilled or deionized water for the OPTION reagent used with the LALS OPTION Height Width Depth Weight 39 1 cm 15 4 in 44 7 ...

Page 45: ...1 2 mm test tube round bottom glass or plastic that fits in the TQ Prep Workstation s carousel Plastic tubes are recommended Mixing Speed 1 200 1 800 rpm Throughput r Three reagent mode 80 tubes per hour r Four reagent mode 64 tubes per hour Sample Processing Time r ImmunoPrep A B and C 38 seconds 2 seconds r ImmunoPrep A B and C and OPTION reagent 47 seconds 2 seconds Cycle Type Reagent A µL Reag...

Page 46: ...The precision reproducibility of the reagent delivery volumes is the coefficient of variation CV as listed below r Reagent A 5 r Reagent B 5 r Reagent C 5 r OPTION Reagent 15 Calibration Gravimetric measurement of delivery volumes density of distilled or deionized water 1 0 g mL The density of ImmunoPrep A and C is insignificantly different from distilled water The density of ImmunoPrep B is 1 04 ...

Page 47: ...PN 4237395AA 4 4 SPECIFICATIONS CHARACTERISTICS PERFORMANCE CHARACTERISTICS ...

Page 48: ...PN 4237395AA A 1 A ALOG SHEETS This Appendix contains these log sheets Page Reagent Log A 3 Maintenance and Operational Checks Log A 5 Action Log A 7 Make photocopies as needed ...

Page 49: ...PN 4237395AA A 2 LOG SHEETS ...

Page 50: ...S A COULTER TQ PREP WORKSTATION REAGENT LOG TQ Prep ImmunoPrep Reagent System Date Opened Lot Number Expiration Date Technician SERIAL NO ___________________________ LAB _________________________________ SCIENCE SERVING HUMANITY ...

Page 51: ...PN 4237395AA A 4 LOG SHEETS ...

Page 52: ...ECKS LOG Daily Every 6 Months or As Needed As Needed Startup QC Check Shutdown Clean Gravimetric Validation Indicate Procedure Date Tech Date Tech Date Tech Procedure Date Tech SERIAL NO _________________________________ LAB _______________________________________ SCIENCE SERVING HUMANITY ...

Page 53: ...PN 4237395AA A 6 LOG SHEETS ...

Page 54: ...SHEETS A COULTER TQ PREP WORKSTATION ACTION LOG Date Condition Noted Tech Initials Date Action Taken Tech Initials SERIAL NO _______________________________ LAB _____________________________________ SCIENCE SERVING HUMANITY ...

Page 55: ...PN 4237395AA A 8 LOG SHEETS ...

Page 56: ...his certification procedure before using the system B 2 OPERATOR CERTIFICATION PROCEDURE 1 Use the Operator Certification form at the end of this appendix to record your results 2 Calibrate a pipet using the appropriate Pipet Calibration Procedure air displacement or positive displacement listed below 3 Use the pipet calibrated in step 2 above to perform the TQ Prep Workstation Precision Procedure...

Page 57: ...ION PROCEDURE PIPET CALIBRATION PROCEDURE With Air Displacement Pipet Reverse Mode 1 Securely fasten a new tip to the pipet 2 Adjust the volume to 100 µL if it is not a fixed volume pipet 3 Tare a weigh boat on a microbalance 1 0 0 1 0 0 ...

Page 58: ...ICATION PROCEDURE PIPET CALIBRATION PROCEDURE B 4 Press the plunger down to the first stop 5 Place the tip 2 to 3 mm below the surface of a container of deionized or distilled water 6 Continue to press the plunger down 2 to 3 mm more ...

Page 59: ...aw the required volume 8 Remove the tip from the deionized or distilled water while touching the tip to an inside wall to remove any adhering liquid 9 While touching the tip to the tared weigh boat slowly dispense the water by depressing the plunger to the first stop only There must be liquid remaining inside the tip ...

Page 60: ...__________mg 9 __________mg 10 _________mg Mean _______mg SD _________mg 1 __________mg 2 __________mg 3 __________mg 4 __________mg 5 __________mg 6 __________mg 7 __________mg 8 __________mg 9 __________mg 10 _________mg Mean _______mg SD _________mg 1 __________mg 2 __________mg 3 __________mg 4 __________mg 5 __________mg 6 __________mg 7 __________mg 8 __________mg 9 __________mg 10 _________...

Page 61: ...ICATION PROCEDURE PIPET CALIBRATION PROCEDURE With Positive Displacement Pipet 1 Securely fasten a new tip to the pipet 2 Adjust the volume to 100 µL if it is not a fixed volume pipet 3 Tare a weigh boat on a microbalance 1 0 0 1 0 0 ...

Page 62: ...FICATION PROCEDURE PIPET CALIBRATION PROCEDURE B 4 Press the plunger all the way down 5 Place the tip 2 to 3 mm below the surface of a container of deionized or distilled water 6 Slowly release the plunger to draw the required volume ...

Page 63: ... the plunger all the way down 9 Record the weight in milligrams on the Operator Certification form at the end of this appendix Part I Pipet Precision Weight of water dispensed Type ____ Air Displacement ____ Positive Displacement 1 __________mg 2 __________mg 3 __________mg 4 __________mg 5 __________mg 1 ____________ 2 ____________ 3 ____________ 4 ____________ 1 ____________mg 2 ____________mg 3...

Page 64: ... __________mg 2 __________mg 3 __________mg 4 __________mg 5 __________mg 6 __________mg 7 __________mg 8 __________mg 9 __________mg 10 _________mg Mean _______mg SD _________mg COULTER TQ PREP Workstation Operator Certification Form 100 1 100 0 100 0 99 8 99 9 100 1 100 0 100 1 100 0 99 8 Part I Pipet Precision Weight of water dispensed Pipet Type ____ Air Displacement ____ Positive Displacement...

Page 65: ...RKSTATION PRECISION PROCEDURE B 4 TQ PREP WORKSTATION PRECISION PROCEDURE 1 Use the pipet calibrated above to perform this procedure 2 Completely mix a tube of normal donor blood by inversion 3 Pipet 100 µL of blood into a 12 x 75 mm test tube 100 µL WHOLE BLOOD ...

Page 66: ...STATION PRECISION PROCEDURE B 4 Place the test tube into the TQ Prep Workstation carousel 5 Repeat steps 2 through 4 until all 32 positions of the carousel are filled 6 Open the sample processing area lid 31 30 20 26 19 21 22 23 2524 15 16 18 17 1 2 3 4 5 7 8 9 32 ...

Page 67: ...ON PROCEDURE TQ PREP WORKSTATION PRECISION PROCEDURE 7 Load the carousel Note To correctly secure the carousel line up and place the arrow on the carousel over the hub key on the indexing base 8 Close the sample processing area lid 9 Press ...

Page 68: ... processing is done remove the carousel and assay the samples for lymphocytes on a flow cytometer per the instrument s manual 11 Acceptable results a The histograms must not show unlysed erythrocytes b All 32 tubes must recover the same percent lymphocytes 2 of the mean using the same analysis region ...

Page 69: ...PN 4237395AA B 14 OPERATOR CERTIFICATION PROCEDURE TQ PREP WORKSTATION PRECISION PROCEDURE ...

Page 70: ..._______________ mg 10 ________________ mg Mean ______________ mg Mean ______________ mg Mean ______________ mg SD ________________ mg SD ________________ mg SD ________________ mg Part II COULTER TQ Prep Workstation Precision Percent Lymphocytes from FS vs SS Histogram 1 __________________ 13 __________________ 25 __________________ 2 __________________ 14 __________________ 26 __________________ ...

Page 71: ...PN 4237395AA B 16 OPERATOR CERTIFICATION PROCEDURE TQ PREP WORKSTATION PRECISION PROCEDURE ...

Page 72: ...d Mortality Weekly Report 38 S 5 1 9 3 Mishell BB and Shiigi AM 1980 Selected methods in cellular immunology San Francisco WH Freeman and Co 4 Caldwell CW and Taylor HM 1986 A rapid no wash technique for immunophenotypic analysis by flow cytometry Am J Clin Path 86 5 600 5 Barker JW 1988 An innovative lymphocyte preparation system for flow cytometry Am Clin Lab 7 7 32 6 Kotylo PK Baenzinger JC Yod...

Page 73: ...PN 4237395AA 2 REFERENCES ...

Page 74: ... distillation Can be used interchangeably with deionized water in these procedures Erythrocyte Red blood cell Flow cytometry A process for measuring the characteristics of cells or other biological particles as they pass through a measuring apparatus in a fluid stream g Gram a unit of weight Gravimetric Of or relating to measurement by weight Histogram light scatter A two dimensional graphic prese...

Page 75: ... its optical sensor detects the presence of an object such as the test tube Positive displacement pipet A pipet that entraps a measured quantity of liquid increases its pressure and then delivers the liquid Precision A measure of the ability of the instrument to reproduce similar results when a sample is run repeatedly Also called reproducibility Preincubation Maintaining a chemical mixture at a s...

Page 76: ...PN 4237395AA 3 GLOSSARY V Volt a unit of electrical potential difference measurement Vac Volts of alternating current Vortex A whirling motion of liquid W Watt a unit of power ...

Page 77: ...PN 4237395AA 4 GLOSSARY ...

Page 78: ...n figure 3 1 tube loading position definition GLOSSARY 2 tube loading position description 3 2 tube loading position figure 3 1 CD4 definition GLOSSARY 1 centrifugation definition GLOSSARY 1 characteristics performance 4 3 checking alignment at installation 2 19 cm definition GLOSSARY 1 coefficient of variation CV definition GLOSSARY 1 components system description 1 2 dimensions 4 1 location 1 2 ...

Page 79: ...metric Validation at installation 2 22 ground path requirement 2 1 H hardware options 1 4 special requirements 2 1 histogram light scatter definition GLOSSARY 1 history of methods 1 1 home position carousel definition GLOSSARY 1 description 3 1 figure 3 1 hub key description 1 2 location on system 1 2 humidity allowance 2 1 4 1 Hz definition GLOSSARY 1 I icons convention used x definition GLOSSARY...

Page 80: ...n GLOSSARY 2 leukocyte definition GLOSSARY 2 level sense connector colors 2 12 how to connect 2 12 location on instrument 2 12 location reagents 2 12 location WASTE 2 12 lid See sample processing area lid See also reagent cover log sheets action A 7 maintenance and operational checks A 5 reagent A 3 lot number definition GLOSSARY 2 lysing definition GLOSSARY 2 lysing reagent 1 4 lysis definition G...

Page 81: ...7 Power Up screen 2 18 precision reproducibility characteristics 4 3 definition GLOSSARY 2 operator certification procedure B 10 specifications 4 3 preincubation definition GLOSSARY 2 preventive maintenance and operational checks log sheet A 5 priming amount of reagent used 4 2 at installation 2 21 automatic 3 3 new reagent 3 3 types 3 2 4 2 user requested 3 2 principles operation 3 1 Q QC quality...

Page 82: ...essing area lid location on system 1 2 sample tube material made of 4 2 size 4 2 See also tube SD standard deviation definition GLOSSARY 2 software screens Power Up screen 2 18 space needed instrument 2 1 special requirements installation 2 1 specifications instrument 4 1 performance 4 3 specimen processing time specifications 4 2 type 4 2 See also sample stabilizing reagent 1 4 standard deviation...

Page 83: ... definition GLOSSARY 3 ventilation requirements 2 1 vortex definition GLOSSARY 3 vortex mixer description 1 3 location on system 1 2 W W definition GLOSSARY 3 WASTE container location in system 2 15 water deionized definition GLOSSARY 1 for use as OPTION reagent 1 5 water distilled definition GLOSSARY 1 for use as OPTION reagent 1 5 worksheet See log sheets ...

Page 84: ...hat it is used on the computer hardware and in the operating system environment for which it was designed Should the media on which your software arrives prove defective Beckman Coulter will replace said media free of charge within 90 days of delivery of the Product This is your sole remedy for any breech of warranty for this software Except as specifically noted above Beckman Coulter makes no war...

Page 85: ......

Page 86: ...mmunoPrep Multi Q Prep Q Prep and TQ Prep are trademarks of Coulter International Corp Teflon is a registered trademark of E I duPont de Nemours Co All other trademarks service marks products or services are trademarks or registered trademarks of their respective holders ...

Page 87: ...rols and Indicators Startup Sample Processing Shutdown Calibration Cleaning Procedures Replace Adjust Procedures Troubleshooting Master Index S Operating Summary In English PN 4237389 Overview of daily procedures and description of screen buttons and icons Come visit us at www beckmancoulter com Beckman Coulter Ireland Inc Mervue Business Park Mervue Galway Ireland 353 91 774068 Copyright Beckman ...

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