389D000 Rev B
7
III - PROPER CABINET USE
CAUTION
A Biological Safety Cabinet is a valuable supplement to, but not a replacement for, good laboratory
technique and safe practice.
If the operator does not operate the cabinet correctly, it may not provide an adequate protective
barrier. To ensure personnel protection, product protection, and environmental protection the
cabinet must be operated per the manufacturer’s instructions.
The facility industrial hygienist or safety officer shall ensure that:
The cabinet is appropriate for all operations and procedures to be performed.
All operators are thoroughly trained and competent regarding cabinet operation and all procedures they
are required to perform.
That the cabinet operation, procedures, and operators are monitored at regular intervals to ensure that
safety is maintained.
A good reference source is The Biosafety In Microbiological and Biomedical Laboratories (BMBL) 5
th
edition
published by the U.S. Department of Health & Human Services as HHS Publication No. (CDC) 21-1112 as an
advisory document for safe work practices.
Baker biosafety cabinets are designed for continuous operation. It is recommended that the blower be left on at
all times to provide containment and keep the interior work area clean and free of particulates.
Operation and Controls
The operator controls with indicators are arranged on the front electrical panel of the cabinet. A number of
switches are arranged in a single membrane switch assembly. [Reference Figure 3]
Figure 3: Operator Controls
Blower
This pushbutton switch controls the cabinet blower. Pressing the Blower pushbutton switch will turn the blower
ON. A green indicator light located below the switch will illuminate when the switch is on. Pressing the Blower
pushbutton switch when the blower is running will turn the blower OFF. If the cabinet blower is turned off, the
Fluorescent Lights will turn off.
ALARM
Alarm Mute / Reset
Sash Level Alarm
Blower
On / Off
Auxiliary Outlets
On / Off
Fluorescent Light
On / Off
UV Light
On / Off
Indicator
Lights
