3
INTRODUCTION
Please read this manual carefully before using the PhO
2
xBox and familiarise yourself with all aspects
of using the workstation. The Baker Company (Baker) or Ruskinn Technology Ltd (Ruskinn) does not
accept responsibility for accidents to personnel or damage to the PhO
2
xBox workstation resulting from
incorrect use.
The PhO
2
xBox comprises of a Gas Controller and a Cell-Culture Chamber has been specifically designed
and developed to meet the requirements of all laboratories.
The system has built in gas control with Oxygen and Carbon Dioxide sensors to enable constant
monitoring of the atmosphere. Hypoxic conditions are created using Nitrogen (N
2
), Carbon Dioxide
(CO
2
) and Compressed Air (O
2
) (or 25% Oxygen in Nitrogen) to achieve the desired atmosphere. The
user can select the Oxygen concentration from 0.1% to 20.9%, and Carbon Dioxide concentrations
from 0.1% to 30.0%.
Many unique features of the PhO
2
xBox workstation are covered in detail in this manual. It is
recommended that the user be fully conversant with the instruction and procedures, and that the
operator familiarises themselves with all aspects and functions of the system before it is commissions
to maintain optimum performance.
