background image

 

22

  

Re-setting the O

and CO

value during operation 

To re-

set the value during operation, press the “stop” button, change the value, and press the 

“start” button. The new value will now become the Set Point.

 

 

Figure 30: Screen 5 

 Atmosphere Control Screen 

Hypoxic Cycle Menu Settings 

The Hypoxic Cycle menu is accessed from Screen 2 to allow for dynamic changing of the Oxygen and 
Carbon Dioxide levels over fixed time periods. Screen 6 shows the initial screen display for 
continuous cycling for up to 4 set-point. 

 

Figure 31: Screen 6 

 Hypoxic Cycle Menu Settings 

The user can set hypoxic cycle on the left side of screen 6. The timing values define how long the 
Oxygen and Carbon Dioxide levels are maintained for once they have been achieved. This means 
that there is a time period between the steps that cannot be set as it is dependent on the difference 
between the set-points within the gas control software.  

Pressing on any of the values will bring up a dialogue box to enter the required setting. If only 2 
values are required for the cycling then set step 1 and 2 as required, then set step 3 times period to 

0 minutes with the O₂ and CO₂ set

-points the same as step 2.  

Once the setting is correct then press the start button to begin the cycling. At this point the start 
button will change to a Stop button. The continuous cycling will stop by pressing the Stop button. 
The left side of the screen is the current status of hypoxic cycle. 

 

 

Summary of Contents for Ruskinn PhO2xBox

Page 1: ...Affix Serial Number Sticker Here User Manual UM 048 PhO2xBox Cell Culture Chamber System PhO2xBox...

Page 2: ...MBER 13 SETTING UP THE CELL CULTURE CHAMBER ON A LABORATORY BENCH 13 FOR INSTALLATION INSIDE A WORKSTATION SCI TIVE OR INVIVO2 OR CO2 INCUBATOR 14 USING THE GAS CONTROLLER 17 THE TOUCH SCREENS 19 STAR...

Page 3: ...igure 18 Front View 17 Figure 19 Left View 17 Figure 20 Right View 17 Figure 21 Rear View 17 Figure 22 Rear View Multi version 18 Figure 23 Top View 18 Figure 24 Screen 1 Logo screen 19 Figure 25 Scre...

Page 4: ...has built in gas control with Oxygen and Carbon Dioxide sensors to enable constant monitoring of the atmosphere Hypoxic conditions are created using Nitrogen N2 Carbon Dioxide CO2 and Compressed Air O...

Page 5: ...em or cause physical injury or damage to the equipment All servicing and repairs must be carried out by a qualified customer service engineer Only genuine spare parts must be used All gas cylinders mu...

Page 6: ...must not be treated as household waste Instead it shall be handed over to an appropriate collection point for the recycling of electrical and electronic equipment If in doubt please return this equip...

Page 7: ...Hazard and actions to be taken in order to avoid the Hazard Warning this equipment contains high voltage circuitry Contains material or substances that may be hazardous to human health Please refer to...

Page 8: ...lled or relocated by a qualified engineer To arrange installation or relocation please contact your local distributor The mains appliance coupler and plug are the AC mains supply isolation device and...

Page 9: ...Electrical Service Requirements Gas Supply Requirements The gas regulator should provide a minimum supply pressure of 2 bar gauge The maximum supply pressure permissible is 3 bar gauge See Table 4 be...

Page 10: ...hich is sucked into the Gas Controller via an Air pump The user can select the Oxygen concentration from 0 3 to 20 0 and the Carbon Dioxide concentration from 0 3 to 20 0 The Cell Culture Chamber is w...

Page 11: ...10 Figure 1 Blue Tube Figure 2 Mains Lead Figure 3 Signal Lead Figure 4 Gas Controller With air pump...

Page 12: ...11 Figure 5 N2 Gas Hose Figure 6 CO2 Gas Hose Figure 7 Cell Culture Chamber Small QTK A01 Figure 8 Cell Culture Chamber Large QTK A02...

Page 13: ...12 Figure 9 Cell Culture Chamber Large QTK AB02...

Page 14: ...Chamber can be placed adjacent to or on top of the Gas Controller Connect the blue tube to InA on the Cell Culture Chamber and OutA on the Gas Controller Connect the signal lead to InB on the Cell Cul...

Page 15: ...wn on Gas Controller Open gas cylinders and turn the gas controller power on For installation inside a workstation SCI tive or InvivO2 or CO2 Incubator Place Cell Culture Chamber in desired Workstatio...

Page 16: ...Additional holes can also be drilled by user Connect the blue tube to InA on the Cell Culture Chamber and OutA on the Gas Controller The blue tube has to be put through the Universal Multi Cable glan...

Page 17: ...he Gas Controller to the N2 gas cylinder and the black hose to connect CO2 on the Gas Controller to the CO2 cylinder Connect the mains lead to the right port shown on Gas Controller Figure 17 Pneumati...

Page 18: ...17 USING THE GAS CONTROLLER Gas Controller The Gas Controller is shown as follows Figure 18 Front View Figure 19 Left View Figure 20 Right View Figure 21 Rear View...

Page 19: ...18 Figure 22 Rear View Multi version Figure 23 Top View...

Page 20: ...en Screen 1 will appear and then will be replaced by the Main Screen Screen 2 Figure 24 Screen 1 Logo screen Main screens From this menu you will be able to access the Main screen below Figure 25 Scre...

Page 21: ...menu is then displayed with the currently active chamber displayed in the top left corner Figure 27 Multi chamber version main screen Atmosphere Control Screen Pressing the Atmosphere Control bar on...

Page 22: ...pear for the new setting to be selected as shown in Screen 4 Press Enter once the desired value of O2 is selected Figure 29 Screen 4 Level SET POINT Screen Repeat for CO2 Once O and CO set points have...

Page 23: ...he timing values define how long the Oxygen and Carbon Dioxide levels are maintained for once they have been achieved This means that there is a time period between the steps that cannot be set as it...

Page 24: ...pear from the system USB Save Press USB Save button and the data log will downloaded to a USB Figure 32 Screen 7 Trend Log Engineer Mode Screen To set the control parameters enter the Engineer Mode sc...

Page 25: ...ng time when the unit enters the intermittent adjustment mode Switch off range 1 20s means rest time when the unit enters the intermittent adjustment mode for example if set switch on at 2s and switch...

Page 26: ...he chamber can withstand up to 60 The Cell Culture Chamber is connected to Gas Controller which contains O and CO sensors A dish of distilled water can be installed in the culture chamber to increase...

Page 27: ...ICULAR PURPOSE THE IMPLIED WARRANTY OF MERCHANTABILITY OR ANY IMPLIED WARRANTY ARISING OUT OF A COURSE OF DEALING OR OF PERFORMANCE CUSTOM OR USAGE OF TRADE Ruskinn Technology Limited DOES NOT WARRANT...

Page 28: ...old waste site Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment Alternatively please contact your local distributor for disposal...

Page 29: ...Sales sales bakerruskinn com Technical support techsupport bakerruskinn com General enquiries ruskinnoffice bakerruskinn com Website www bakerruskinn com YouTube channel http www youtube com ruskinnte...

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