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INTRODUCTION
Please read this manual carefully before using the InvivO
2
and familiarise yourself with all
aspects of using the workstation. The Baker Company (Baker) or Ruskinn Technology Ltd
(Ruskinn) does not accept responsibility for accidents to personnel or damage to the
InvivO
2
workstation resulting from incorrect use.
The InvivO
2
has been specifically designed and developed to meet the requirements of all
laboratories. The unit is ergonomically laid out to ensure maximum operator comfort and
optimization of space for media preparation, manipulation, and analysis. InvivO
2
is
designed to give optimum ‘in-vitro’ conditions for cell culture in a simple and easily
controlled manner.
The system has built in gas control with Oxygen and Carbon Dioxide sensors to enable
constant monitoring of the atmosphere. Hypoxic conditions are created using Nitrogen
(N
2
), Carbon Dioxide (CO
2
) and Compressed Air (O
2
) (or 25% Oxygen in Nitrogen) to achieve
the desired atmosphere. The user can select the Oxygen concentration from 0.1% to
20.9%, and Carbon Dioxide concentrations from 0.1% to 30.0%.
A Heater system maintains the temperature within the workstation between a5°C
to 45°C, which is set by the user. The cooling accessory for the workstation will achieve an
unloaded temperature of 15°C or less which is set by the user.
Optional humidity generation system allows the maintenance of Relative Humidity levels
from ambient to +85% RH @ 37°C which is user settable. The generation of humidity is
either through nebulisation which promotes evaporation from a body of water by breaking
the surface tension or through steam generation.
Many unique features of the InvivO
2
workstation are covered in detail in this manual. It is
recommended that the user be fully conversant with the instruction and procedures, and
that the operator familiarises themselves with all aspects and functions of the system
before it is commissions to maintain optimum performance.
