Allsheng Auto-Pure 20A Operation Manual Download Page 1

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Operation Manual

V3.0

Auto-Pure Series

Nucleic Acid Purification

System

Hangzhou Allsheng Instruments Co., Ltd.

Summary of Contents for Auto-Pure 20A

Page 1: ...Operation Manual V3 0 Auto Pure Series Nucleic Acid Purification System Hangzhou Allsheng Instruments Co Ltd ...

Page 2: ...lease check the instrument and Appendix with the packing list when you first open the package If you find anything missing or incorrect please contact the distributor HANGZHOU ALLSHENG INSTRUMENTS CO LTD Address Building 1 2 Zheheng Science Park Zhuantang Town Xihu District Hangzhou Zhejiang 310024 China Telephone 86 571 88802738 88948289 E mail info allsheng com Website www allsheng com File No A...

Page 3: ...r use of the instrument may cause damage to the system inaccurate results or potentially nullify warranties Operation without reading the manual may cause damage or even electrical shock Indoor use only Read the Manual carefully before operation only qualified and The operator should not open or repair the Instrument without Vendor s authorization if not there might be cause potential damages or i...

Page 4: ...amaged Please make sure there s nothing covered the power cord and keep it away from crowds when in use Power off the instrument after operation and please disconnect the plug if long time no use of the instrument and cover it with something to prevent from dust Under the following circumstances please disconnect the power immediately and contact with your distributor Liquids into the Instrument D...

Page 5: ...y the cloth with alcohol If there are any stains on the Instrument clean them with cleansing cream 4 The limiting condition of transportation and storage environment Ambient temperature range 10 35 Relative humidity 70 Atmosphere range 500 1060hpa No corrosive gas and a well ventilated room ...

Page 6: ...mension 3 Chapter 3 Product Introduction 4 1 Structure 4 2 Operation panel 6 Chapter 4 Operation 7 1 Preparation 7 2 Connect the power 8 3 Install the reagent kit 8 4 Install the magnetic rod cover 10 5 Remove magnetic rod cover 11 6 Operation 12 Chapter 5 Troubleshooting 29 Chapter 6 Spare Parts List 31 Chapter 7 Abbreviation and Symbols 32 ...

Page 7: ... special reagent kit or 96 well reaction plate and when using the different reagent would be able to extract DNA RNA from animal or plant tissue blood and body fluid etc Features Friendly interface with easy operation Touch screen with 3 shortcut key or mouse operation Heating function in Lysis and elution UV steriliza tion Quiet operation without vibration One stop operation to avoid contaminatio...

Page 8: ...ml tubes 5ml Kits 2ml tubes Sample Volume μL 50 1000 50 1000 50 3000 50 5000 50 10000 50 5000 Stability CV 5 Lysis temp Ambient temperature 120 Elution temp Ambient temperature 120 Heating time Heating time Ambient temperature 120 4 minutes Temperature Accuracy 1 Operation interface 7 inch touch screen 3 shortcut buttons and mouse is available Built in protocol 8 groups of preset protocols 100 gro...

Page 9: ...mm 470mm 450mm 400mm 520mm 450mm Weight kg 28kg 30kg 3 Overall Dimension Dimension W D H Auto Pure 32A 400mm 470mm 450mm Auto Pure 48A 400mm 470mm 450mm Auto Pure 20A 400mm 520mm 450mm Auto Pure 20B 400mm 520mm 450mm Auto Pure 24D 400mm 520mm 450mm Auto Pure 24BT 400mm 520mm 450mm ...

Page 10: ...roduce the instrument structure operation buttons display panel as well as the preparations before operate For the first time user please make sure to read this chapter before start 1 Structure 1 1 Front 1 2 Back Display screeen Shortcut key Operation Window Socket Fuse Power Switch USB port Ethernet port Debuggling port Fan ...

Page 11: ... heating strip 1 4 Auto Pure 32A 48A Inside view Note The only difference between the experiment module of above products is the shape of the heating strip Magnetic rod Over the magnetic rod cover Magnetic rod cover stand Drawer Heating strip UV light top inside Magnetic rod Over the magnetic rod cover Fan bottom inside Magnetic rod cover stand Drawer Heating strip UV light top insid Fan bottom in...

Page 12: ...ter 3 Product Introduction 6 2 Operation panel Display screen Operate by touch screen or mouse which connect with USB port TAB Select for the shortcut program RUN Run for the shortcut program STOP Stop operating Display screen TAB RUN STOP ...

Page 13: ...arton and tear off the tape on the edge of operation window then open it and take out the foam First please screw out the fixed screw of position as below Second please screw out the screw of position then you can take out the baffle and release the moving component Note Be careful in operating or the magnetic rod will be broken ...

Page 14: ... on the heating strip make sure the sample well marked with 1 should be on the left side then push back the drawer slowly Note The shape of customized reagent kit of above products is different while the installation is the same 3 2Install the customized reagent kit for Auto Pure 24D 24BT Open the drawer and place the kits with sample on the kits tray then put the kits tray to the drawer and press...

Page 15: ...on plate on Auto Pure 32A Take out the drawer and put the 96 well reaction plate which already filled with samples mounted on the locating slot make sure the plate with chamfering should be on the left side then push back the drawer slowly Customized reagents kits Kits tray drawer door ...

Page 16: ... 4 Install the magnetic rod cover 4 1Install the magnetic rod cover for 20A 20B 24D 24BT Insert the magnetic rod cover completely on the mounting groove and the installation quantity depends on the reagent kit number 96 well reaction plate Magnetic rod cover ...

Page 17: ...mpletely on the mounting groove and the installation quantity depends on the reagent kit number Note Magnetic rod cover for Auto Pure32A at most is 4pcs for Auto Pure at most is 6pcs The installation method is same 5 Remove magnetic rod cover Press the button and take out the magnetic rod cover as per below photo Auto Pure 32A Magnetic rod cover ...

Page 18: ...l Chapter 4 Operation 12 6 Operation 6 1Start up Interface Turn on the instrument and make sure the drawer is closed before start the screen will display the start up interface After start it will enter into the shortcut mode as below ...

Page 19: ...6 3Shortcut mode Under the shortcut mode select the program needed and click Run it will enter into the program run interface The program can also be selected by press Tab button on the panel and then press Run for start or Stop for terminate Auto Pure 20A Running interface ...

Page 20: ...Auto Pure series Operation Manual Chapter 4 Operation 14 Auto Pure 20B Running interface Auto Pure 24BT Running interface ...

Page 21: ...Auto Pure series Operation Manual Chapter 4 Operation 15 Auto Pure 24D Running interface Auto Pure 32A Running interface ...

Page 22: ...so left time display on the top right corner Click Pause to pause or continue the program Click Stop the program will stop and there will be check box Click Cancel program will be continue and click OK the program run will be back to original place and the Stop button will change to Run Again Click Run Again to continue the program Click Back to return the previous menu Remark If the drawer is ope...

Page 23: ...eration Manual Chapter 4 Operation 17 6 3 1 List mode Users may review the file by scroll bar on the right side Select the program and click Run to enter into the run interface Click View to enter into the check interface ...

Page 24: ... view the parameter setting of the program Click Back to previous interface 6 3 2 Lamp At the bottom of interface the icon shows that the lamp is on and the icon shows that the lamp is off Click this icon to change the state of the lamp 6 4Program Management Click Manage prog into the surface of program management ...

Page 25: ...Auto Pure series Operation Manual Chapter 4 Operation 19 ...

Page 26: ...layed in the Shortcut list interface In the list of Lock if the icon is the program can not be edited deleted and saved as if the icon is the program can be edited deleted and saved as 6 4 2 Program Management Insert Click Insert under the Manage prog to enter into the Insert surface When new program you should input the name of program in the first place ...

Page 27: ...If input 0 for the position of well insert step is pause then only the name of step is able to edit rest of parameters cannot be edit Under this directive magnetic rod and magnetic rod cover combined and rise If input 9 for the position of well inset step is pause then only the name of step is able to edit rest of parameters cannot be edit Under this directive magnetic rod and magnetic rod cover r...

Page 28: ...7 or below then the device won t heating when running to this step When selecting other wells location than the above the corresponding step line temperature number box is not available Click to enter the extended parameter setting interface it isn t necessary to set in normally use or you can reset if have special requirement Click back to the parameter setting interface Click Delete and then cli...

Page 29: ...Management Save as Under the Manage prog interface to make selection Click Save as and then input a new program name click Enter to save the current program or ESC to not save 6 4 5 Program Management Delete Under the Manage prog interface to make selection Click Delete and then click Ok to confirm the delete or Cancel to not delete 6 5 System Setting Click Settings and enter into the System Setti...

Page 30: ...need to set unless it s failed and need repair as instrument has already finished setting before factory dispatch and even if it s failed this kind setting will be authorized by distributor or manufacturer 6 5 2 System Setting System Time Click Date Time to set system time by directly enter into number or click ...

Page 31: ...e series Operation Manual Chapter 4 Operation 25 6 5 3 System Setting Language Click Language Setting to choose the language that you need 6 5 4 System Setting Fan Click Air Ejector Fan to have the fan setting ...

Page 32: ...ation 26 6 5 5 System Setting Import Export Click Import Export and insert U disk to finish the step 6 5 6 System Setting Software upgrade Click Software upgrade to input the right password enter the interface and then insert the U disk to operation ...

Page 33: ...Click UV sterilization and input number or click to set time Click Start to open the UV light to start UV sterilization and time count down Click Stop to stop the UV sterilization During sterilization the UV light will automatically stop when the drawer is open and it will continue ...

Page 34: ...Auto Pure series Operation Manual Chapter 4 Operation 28 after the drawer is closed 6 6 Help Click Help to check the help info Help interface displays the relevant features and version information ...

Page 35: ...ight tube Contact with distributor 4 Instrument not able to automatically stop after drawer is open Sensor failure Contact with distributor 5 Big variance between actual and display temperature Sensor failure Contact with distributor 6 No heating in heating strip Sensor failure Contact with distributor SCR failure Heater failure 7 Instrument can t run Controller failure Contact with distributor Mo...

Page 36: ...24D Auto Pure 24BT T1 T2 T3 T4 Drive circuit fault E018 E028 E038 E048 T1 T2 T3 T4 Open circuit E015 E025 E035 E045 T1 T2 T3 T4 Short circuit E016 E026 E036 E046 The drive circuit of exhaust fan fault E019 Auto Pure 20A Auto Pure 20B Auto Pure 24D Auto Pure 24BT Auto Pure 32A Auto Pure 48A The drive circuit of cooling fan fault E009 Electric machinery code 1 Electric machinery brake lock fault E10...

Page 37: ...er 6 Spare Parts List 1 Auto Pure 20A 20B Spare parts list No Item Unit Qty Remark 1 Power line pc 1 2 Mouse pc 1 3 Kits tray pc 2 4 Allen wrench pc 1 2 Auto Pure 32A 48A 24D 24BT Spare parts list No Item Unit Qty Remark 1 Power line pc 1 2 Mouse pc 1 3 Allen wrench pc 1 ...

Page 38: ... Symbols 1 Abbreviation Abbreviation used A Ampere AC Alternating current V Volt Hz Hertz W Watt USB Universal Serial Bus SD Secure Digital Card WiFi WLAN kg Kilogram mm Millimeter μL Microlitre hPa Hectopascal Degree Centigrade CV Coefficient of variation of well TAB Switch RUN Operation STOP Stop ...

Page 39: ...n Manual Chapter 7 Abbreviation and Symbols 33 2 Symbols Symbols used on device Warning Heating Indicates conformity with health safety and environmental protection standards for products sold within the European Economic Area ...

Page 40: ...Auto Pure series Operation Manual Notes 34 Notes ...

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