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5
Agilent DNA 7500 and DNA 12000 Assay Protocol
Loading the Ladder and the Samples
Loading the Ladder and the Samples
1
Pipette 1 μl of the yellow-capped ladder vial
(yellow ) in the well marked with the ladder
symbol .
2
In each of the 12 sample wells pipette 1 μl
of sample (used wells) or 1 μl of deionized water
(unused wells).
3
Set the timer to 60 seconds.
4
Place the chip horizontally in the adapter of the IKA vortex mixer and make
sure not to damage the buldge that fixes the chip during vortexing.
5
Vortex for 60 seconds at 2400 rpm.
6
Refer to the next topic on how to insert the chip in the Agilent 2100
Bioanalyzer. Make sure that the run is started within 5 minutes.
OODGGHU
OVDPSOH
N O T E
For optimal results, samples should be of pH 6 to 9 and should not have an ionic content
greater than twice that of a typical PCR buffer.
C A U T I O N
Wrong vortexing speed
If the vortexing speed is too high, liquid spill that disturbs the analysis may occur for
samples generated with detergent containing buffers.
➔
Reduce vortexing speed to 2000 rpm!
