13
Agilent DNA 7500 and DNA 12000 Assay Protocol
5
Preparing the Gel-Dye Mix
2
Vortex the blue-capped vial with DNA dye
concentrate (blue ) for 10 seconds and
spin down. Make sure the DMSO is
completely thawed.
3
Pipette 25 μl of the blue capped dye
concentrate (blue ) into a red-capped
DNA gel matrix vial (red ). Store the dye
concentrate at 4 °C in the dark again.
4
Cap the tube, vortex for 10 seconds. Visually inspect proper mixing of
gel and dye.
5
Transfer the complete gel-dye mix to the top receptacle of a spin filter.
6
Place the spin filter in a microcentrifuge and spin for 10 minutes at
room temperature at 1500 g ± 20 % (for Eppendorf microcentrifuge, this
corresponds to 4000 rpm).
7
Discard the filter according to good laboratory practices. Label the tube
and include the date of preparation.
N O T E
Always use the volumes indicated. Using different volumes in the same ratio will produce
inaccurate results.
N O T E
The prepared gel-dye mix is sufficient for 10 chips. Use the gel-dye within 4 weeks of
preparation.
Protect the gel-dye mix from light. Store the gel-dye mix at 4 °C when not in use for more
than 1 hour.
