Agilent Technologies DNA 12000, Manual Kit

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Agilent DNA 7500 and DNA 12000 Kit
1
Table 1 Physical Specifications
Type Specification
Analysis time 30 minutes
Samples per chip 12
Sample volume 1 µL
Kit stability 4 months (see box for storage temperatures)
Kit size 12 samples/chip = 300 samples/kit
Table 2 Analytical Specifications
Type Agilent DNA 7500 Agilent DNA 12000
Sizing range 100 – 7500 bp 100 – 12000 bp
Sizing resolution 100 – 1000 bp: 5 %
1000 – 7500 bp: 15 % 100 – 1000 bp: 5 %
100 – 12000 bp: 15 %
Sizing accuracy ± 10 % ± 15 %
Sizing reproducability 5 % CV 5 % CV
Quantitation
accuracy
1
1
Determined using the respective DNA ladder as sample
20 % 25 %
Quantitation
reproducibility
1
100 – 1000 bp: 10 % CV
1000 – 7500 bp: 5 % CV 100 – 1000 bp: 15 % CV
1000 – 12000 bp: 10 % CV
Quantitative range
1 0.5 – 50 ng/µl 0.5 – 50 ng/µl
Maximum salt 250 mM for KCl
15 mM for MgCl
2
250 mM NaCl
250 mM for KCl
15 mM for MgCl
2
250 mM NaCl