Agilent Technologies AdvanceBio Amino Acid Analysis, User Manual

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Important Safety Considerations
• All points of connection in liquid chromatographic systems are
potential sources of leaks. Users should be aware of the toxicity or
flammability of their mobile phases.
• Because of the small particle size, dry column packings are respirable.
Agilent does not recommend removing the column end fittings and
exposing the media. Columns should only be opened by trained
personnel in a well-ventilated area.
• Please adhere to operating pressure limits noted for each column
(see Table 1 ). Exceeding these limits will compromise
chromatographic performance and column lifetime, and could
be unsafe.
Other Operating Tips
• While generally not harmful to the column, reverse flow
should be avoided except to attempt removal of clogged frit
(see “column care”).
• It is recommended that the flow rate is started at a reduced rate
and then gently increased to the desired operating flow rate.
• Always use high purity reagents and chromatography grade
solvent to prepare your mobile phase. Degas and filter all mobile
phase prior to use.
• Disassembling a column will degrade column performance.
• An inline filter or guard column may be used to protect your column
and increase its lifetime.
• If the column is used outside of recommended pH ranges for column
phase (see Table 2 ), a reduced lifetime will result.
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