EXI-600 INVERTED MICROSCOPE SERIES
ACCU-SCOPE
®
73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com
37
6.2.2.
Reflected Light Methods
6.2.2.1.
Fluorescence observation – Instrument configuration
Component
Position
Transmitted illumination shutter
Off (closed), no light is coming out of the bottom of the
condenser
Reflected light shutter
On (open)
Reflected light field diaphragm
Fully open
Reflected light aperture diaphragm Fully open
Neutral density filter slider
Open position
Fluorescence light source
On and shutter open
Upper light path selector
Lower light path selector
Condenser turret position
Any position. Condenser is not being used during
fluorescence observation. It is recommend to tilt the light pillar
to the back position to avoid reflection and autofluorescence of
the transmitted LED light source.
Magnification changer
1X
Fluorescence cube turret (if
installed)
Choose an appropriate filter cube for the specimen
Objective(s)
Any, as provide appropriate magnification of the specimen
1.
Confirm that fluorescence excitation illumination is coming from the objective.
2.
Place a specimen on the stage.
3.
Focus on the specimen using the focus knobs.
4.
Change fluorescence cube turret position to a different filter cube and observe again.
5.
If desired, change side port light path selector or trinocular tube upper light path selector to a
camera port for imaging.
