TMA Grand Master 2.1 – User’s Guide
5 Using TMA Grand Master
When recipient (or cleaning) blocks, donor blocks, and PCR cassettes are inserted, the transfer
process can be started.
NOTE:
If using TMA Grand Master in PCR mode only, and if necessary, it is available for you to insert
cleaning blocks into empty slots during the process without interrupting it.
Before starting the transfer process follow the sequence of steps below to place markers on donor
blocks from which locations cores will be extracted to the selected recipient positions or PCR tubes.
1. To place a PCR marker on a donor block, first click the PCR button on the left side of the
donor image. The number of PCR markers placed on the donor block appears in the PCR
button.
Place TMA markers on desired areas of the donor block where tissue core should be
extracted from, then click the position of the recipient block layout that you want to fill with
the core sample.
Important!
Each donor position has its adherent PCR position (for example, A6–PCR6) and because
of the maximum number of PCR markers allowed to place on a donor block is four,
each PCR tube can contain up to four cores of the corresponding donor block. A PCR
cassette (when loaded with 10 PCR tubes) is capable of receiving 40 cores total.
Important!
It is available for you to import markers already placed on the virtual slide.
April 8, 2014 – Rev. 1
3DHISTECH Ltd.
44(79)
